268 GERM CELL DEVELOPMENT IN EQUINE TESTIS TISSUE XENOGRAFTED INTO MICE
Notice bibliographique
Résumé
Grafting of testis tissue from immature animals under the back skin of immunodeficient mice results in complete spermatogenesis, albeit with different levels of efficiency in different species. While spermatogenesis develops comparably to that in the donor species in xenografts from pigs, sheep and goats, spermatogenic differentiation is less efficient in testis tissue from cats and bulls. Testicular maturation was significantly accelerated in rhesus monkey testis grafts whereas timing was similar to that in the donor species in cats and bulls. The objective of this study was to investigate if grafting of immature horse testis tissue would result in spermatogenesis in a mouse host. Small fragments of testis tissue (about 1 mm3) from four sexually immature colts (2-week-old Standardbred, 5- and 8-month-old ponies, 10-month-old Warmblood) were grafted under the back skin of castrated male immunodeficient mice (n = 5, 5, 10 and 5 recipient mice, respectively). Histological examination of the testis xenografts was performed between 14 and 50 week post-transplantation. Weight of the seminal vesicles in the host mouse was recorded as an indicator of bioactive testosterone produced by the xenografts. At the time of grafting, the seminiferous cords of the donor testis tissue form 2-week-, 5-month- and 8-month-old colts contained only immature Sertoli cells and gonocytes. No spermatogenic differentiation occurred in xenografts from the 2-week-old colt and testosterone production was minimal. Pachytene spermatocytes were observed in testis grafts from the 5- and 8-month-old donors from 14 weeks onward. Spermatogenesis did not proceed through meiosis in grafts from the 5-month-old donor. Recipient mice carrying xenografts from the 8-month-old donor received exogenous gonadotropins (equine chorionic gonadotropin and human chorionic gonadotropin, 10 I.U./day for 2 months, beginning 14 weeks after grafting) and condensing spermatids were observed by 35 weeks after grafting. In donor tissue from the 10-month-old colt, pachytene spermatocytes were present in about 50% of tubules at the time of grafting. After 14 weeks, xenografts showed fewer differentiated germ cells than the donor tissue. However, at 35 weeks after grafting, condensing spermatids were observed, indicating that differentiated germ cells were initially lost and spermatogenesis was subsequently reinitiated. In all castrated host mice where spermatogenic differentiation occurred, the weight of the seminal vesicles was restored to pre-castration values showing that xenografts were releasing bioactive testosterone. These results indicate that horse spermatogenesis can occur in a mouse host albeit with low efficiency. Testicular maturation was not accelerated. In most cases, spermatogenesis appeared to become arrested at meiosis. The underlying mechanisms of this spermatogenic arrest require further investigation. Although equine testis xenografts produced testosterone, supplementation of exogenous gonadotropins might support post-meiotic differentiation. This work was supported by USDA 03-35203-13486.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».