138 THE EFFECT OF SYNTHETIC HYALURONAN, BSA AND SERUM ON IN VITRO DEVELOPMENT AND GENE TRANSCRIPTION OF BOVINE BLASTOCYSTS
Notice bibliographique
Résumé
The objective of this study was to examine the effect of synthetic hyaluronan (s-HA), BSA and fetal calf serum (FCS) on bovine embryo in vitro development, ultrastructure, and mRNA transcription of four developmentally important genes: apoptosis (BAX), oxidative stress (SOX), growth factor (IGF-II), and cell-to-cell adhesion (Ecad). A total of 1406 presumptive zygotes (7 replicates) were cultured initially in two Groups: 1, SOFaa + 4% BSA only, and 2, SOFaa + 4% BSA and 10% FCS. On Day 4 (96 h after insemination) of culture, the number of zygotes that developed to the <8-cell-stage were recorded, and 2.5 mg/mL of s-HA (MAP-5; Bioniche Inc, Belleville, ON, Canada) was added to half of the embryos from each group; the other half received an equivalent volume of corresponding SOF medium without s-HA. Embryos were cultured in 50-µL drops (25 zygotes per drop) under paraffin oil at 39°C and 5% CO2 in humidified air. Cleavage rates were recorded on Day 2 and the number of blastocysts on Days 7, 8, and 9. At least five blastocysts from each replicate and from each treatment were frozen for evaluation of gene expression patterns. Poly(A) mRNA was prepared from 4–5 groups of pools of 10 embryos. The quantification of all gene transcripts was performed by real time quantitative RT-PCR in three replicates. The fine structure of blastocysts was studied using transmission electron microscopy. Embryo developmental stages and blastocyst formation were analyzed by chi-square analysis, and data on mRNA expression were analyzed by one-way repeated-measures ANOVA. No differences in cleavage rates were observed between groups. There was no difference between the BSA group with or without s-HA in the percentages of embryos developed to the blastocyst stage at Days 7, 8, and 9 (38.3 and 38.1%, respectively). However, significantly (P < 0.05) less blastocysts developed in medium supplemented with BSA + FCS (18.3%) or with BSA + FCS + s-HA (27.4%). Synthetic HA added to the medium containing BSA significantly (P < 0.05) increased the level of expression of EGF-II and decreased (P < 0.05) the level of expression of BAX, SOX, and Ecad. On the other hand, presence of FCS significantly (P < 0.05) increased the level of SOX and decreased the level of IGF-II (P < 0.05), and the addition of s-HA to SOF containing FCS showed no effect on the level of transcription of any analyzed genes. The general fine structure of embryos cultured with s-HA regardless of protein supplement was conspicuously improved in comparison with the respective controls. It can be concluded that, within our culture system, addition of s-HA on Day 4 of culture to the SOFaa medium supplemented with BSA but not in combination with FCS showed a positive effect on embryo development and molecular composition of the embryos.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».