267 TESTIS TISSUE XENOGRAFTING AS A BIOASSAY FOR GERM CELL DEVELOPMENTAL POTENTIAL IN EQUINE CRYPTORCHID TESTES
Notice bibliographique
Résumé
In domestic animals, spermatogenic differentiation is blocked in abdominally retained testes exposed to core body temperature. It is not known if undifferentiated germ cells are retained in long-term cryptorchid equine testes, nor is it known whether any surviving germ cells retain their ability to progress through spermatogenesis. If functional germ cells do persist in equine abdominal testes, then the possibility exists that offspring could be derived even from bilaterally cryptorchid individuals. Previously, we reported an in vivo model where completion of spermatogenesis with production of spermatozoa capable of fertilization occurred in fragments of testicular tissue from immature mice, domestic animals, and monkeys grafted under the skin of immunodeficient mice. Therefore, spermatogenic development in testis tissue xenografts can serve as an in vivo assay system for the developmental potential of germ cells. The objective of this study was to investigate if cryptorchid horse testes that had been exposed to core body temperature for 1–3 years had retained developmentally competent germ cells. Small fragments of abdominally cryptorchid testis tissue (about 1 mm3) from three donor horses (1-, 2-, and 3-year-old Quarterhorse) were grafted under the back skin of castrated male immunodeficient mice (n = 8, 6, and 3 recipient mice, respectively). At the time of grafting, donor tissue did not contain differentiated germ cells. Histological examination of the testis xenografts was performed between 5 and 45 weeks post-transplantation. Weight of the seminal vesicles in the host mouse was recorded as an indicator of bioactive testosterone produced by the xenografts. By 28 weeks after grafting, pachytene spermatocytes were observed in xenografts from all cryptorchid donor testes. While haploid gametes would be expected to be present in xenografted testis tissue from descended equine testes by 35 weeks after grafting, spermatogenesis did not progress through meiosis in the cryptorchid grafts. In all recipient animals where spermatogenic differentiation occurred, the weight of the seminal vesicles in the castrated host mice was restored to pre-castration values, indicating that xenografts were capable of releasing biologically active testosterone. These results indicate that even after 3 years of exposure to core body temperature, equine cryptorchid testes contain germ cells capable of differentiation. It remains to be investigated if supplementation of exogenous gonadotropins might support post-meiotic differentiation of germ cells in cryptorchid equine testes xenografts. This work was supported by USDA 03-35203-13486.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».