Bone Morphogenetic Protein 15 (BMP15) and Fibroblast Growth Factor 10 (FGF10) Stimulate Cumulus Expansion and Differently Regulate Transcription of Expansion Inducing Genes in Bovine Cumulus Cells.
Notice bibliographique
Résumé
The oocyte actively participates in the regulatory mechanisms of cumulus-oocyte complex (COC) maturation via secretion of paracrine factors. BMP15 and FGF10 were localized to the oocyte and improved blastocyst rates following IVM/IVF in cattle. In this study we tested the hypotheses that FGF10 and BMP15 would enhance cumulus expansion and that the mechanisms would include the transcriptional control of epidermal growth factor (EGF)-like family members [amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC)] and of major expansion-inducing genes [cyclooxygenase 2 (COX2), hyaluronan synthase 2 (HAS2), pentraxin 3 (PTX3), tumor necrosis factor-stimulated gene-6 protein (TSG6)]. Immature COCs (grades 1 and 2) were obtained from 2-8 mm follicles from abattoir ovaries. Groups of 20 COCs were matured for 4, 12, and 22 hours with grading doses of FGF10 (0, 0.5, 10 and 50 ng/ml; n=4) or BMP15 (0, 10, 50 and 100 ng/ml; n=4). After culture for 22 hours, cumulus expansion was visually assessed (grades 1, 2 and 3). To assess of treatments on gene expression, cumulus cells were mechanically separated from COCs after 4, 12 and 22 hours of culture and stored at -80°C. Total RNA was extracted using RNeasy (Qiagen), and 100 ng of RNA was reverse-transcribed. Expression of target genes was assessed by real time RT-PCR and normalized by cyclophilin (CYC-A) expression. Relative mRNA abundance was determined by the Pfaffl equation. Effects of FGF10 and BMP15 treatment were tested by ANOVA and groups were compared by Tukey-Kramer HSD test. Non parametric analysis was used when data were not normally distributed. Both FGF10 (10 ng/ml) and BMP15 (100ng/ml) increased the proportion of COC with fully expanded cumulus (grade 3). FGF10 did not affect the expression of EGF-like family members, but at 10 ng/ml stimulated mRNA abundance of COX2 (4 and 12 hours), PTX3 (12 and 22 hours) and TSG6 (22 hours). In contrast, BMP15 (100 ng/ml) increased mRNA expression of AREG and EREG from 12 hours of culture, before stimulating the expression of COX2, PTX3 and TSG6 at 22 hours of culture. HAS2 mRNA expression was stimulated by BMP15 treatment (100 ng/ml) only at 12 hours of culture in association with the onset of AREG and EREG upregulation. In conclusion, both FGF10 and BMP15 enhanced cumulus expansion in cattle, but induced different gene expression patterns in cumulus cells. While FGF10 increased COX2 promptly and PTX3/TSG6 subsequently without altering AREG and EREG, the increases in COX2, PTX3, TSG6, HAS2 expression induced by BMP15 were observed only after or in association with the increase in AREG and EREG expression. This observation suggests the BMP15 stimulates the expression of expansion-inducing genes via the EGF-like family members, while FGF10 appears to act upon more directly pathways.Supported by FAPESP. (poster)
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».