The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
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Résumé
To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).
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La notice
- Revue
- Genetics
- Thématique
- CRISPR and Genetic Engineering
- Domaine
- Biochemistry, Genetics and Molecular Biology
- Établissements canadiens
- —
- Organismes subventionnaires
- Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNational Center for Research ResourcesCancer Research UKNIH Office of the DirectorNational Key Research and Development Program of ChinaMinistry of Science and Technology of the People's Republic of ChinaNational Natural Science Foundation of ChinaNational Institute of General Medical SciencesSchool of Medicine, New York UniversityInstitute of GeneticsNational Institutes of HealthYale UniversityCold Spring Harbor LaboratoryYork UniversityHoward Hughes Medical Institute
- Mots-clés
- RNA interferenceBiologyGeneticsComputational biologyStock (firearms)PhenotypeGenomeGeneEngineeringRNA
- Résumé présent dans OpenAlex
- oui