Arachidonic Acid Regulates the Translocation of 5-Lipoxygenase to the Nuclear Membranes in Human Neutrophils

Elevation of the intracellular cAMP concentration in agonist-activated human neutrophils (PMN) leads to the concomitant inhibitions of arachidonic acid (AA) release, 5-lipoxygenase (5-LO) translocation, and leukotriene (LT) biosynthesis. We report herein that exogenous AA completely prevents cAMP-dependent inhibition of 5-LO translocation and LT biosynthesis in agonist-activated PMN. Moreover, the group IVA phospholipase A2 inhibitor pyrrophenone and the MEK inhibitor U-0126 inhibited AA release and 5-LO translocation in activated PMN, and these effects were also prevented by exogenous AA, demonstrating a functional link between AA release and 5-LO translocation. Polyunsaturated fatty acids of the C18 and C20 series containing to the were AA in 5-LO translocation in agonist-activated PMN. the inhibitor and the intracellular that the 5-LO translocation and the and 5-LO and AA to the pyrrophenone inhibition of 5-LO translocation, that the of of 5-LO translocation by AA in the of the report that AA the translocation of 5-LO in human and a of the inhibition of LT biosynthesis. Elevation of the intracellular cAMP concentration in agonist-activated human neutrophils (PMN) leads to the concomitant inhibitions of arachidonic acid (AA) release, 5-lipoxygenase (5-LO) translocation, and leukotriene (LT) biosynthesis. We report herein that exogenous AA completely prevents cAMP-dependent inhibition of 5-LO translocation and LT biosynthesis in agonist-activated PMN. Moreover, the group IVA phospholipase A2 inhibitor pyrrophenone and the MEK inhibitor U-0126 inhibited AA release and 5-LO translocation in activated PMN, and these effects were also prevented by exogenous AA, demonstrating a functional link between AA release and 5-LO translocation. Polyunsaturated fatty acids of the C18 and C20 series containing to the were AA in 5-LO translocation in agonist-activated PMN. the inhibitor and the intracellular that the 5-LO translocation and the and 5-LO and AA to the pyrrophenone inhibition of 5-LO translocation, that the of of 5-LO translocation by AA in the of the report that AA the translocation of 5-LO in human and a of the inhibition of LT biosynthesis. LT cAMP IVA phospholipase fatty acid cAMP IVA phospholipase fatty acid of in and and of LT and of LT biosynthesis and the release of AA by the AA by the 5-LO to the of to by and in the of the a and of human LT biosynthesis in of the the the of 5-LO that 5-LO the to to by 5-LO to that 5-LO the to to the that 5-LO a to the and the of the to and the translocation of the in the of 5-LO and translocation to the of 5-LO in functional to LT biosynthesis in of in LT biosynthesis and that a AA and acid to the Moreover, and to LT biosynthesis the translocation of 5-LO to in activated a of and of 5-LO by and of 5-LO and also in the of 5-LO LT in in the of 5-LO translocation to the in activated by and and that intracellular cAMP concentration and that also inhibited the release of AA in activated human that the translocation of 5-LO to the the release of AA in human the AA a in the of 5-LO in human acid acid acid acid acid acid acid acid acid acid and were AA were and and were 5-LO and were a of a of MEK and were and were of and were the and the were by were the by the to the the were in containing were were and were of were were of AA release by the inhibitor were and pyrrophenone and were of the the of LT biosynthesis by in were by the of of and were and were the of 5-LO and and AA the of 5-LO and AA, were of a containing of and AA the also of were to the and the were and by the of and and and and to 5-LO AA the were to a and in of by in the of 5-LO and were of and and to the and and were to were by were and were by were in containing 5-LO and a and the 5-LO and that the of LT biosynthesis in inhibition of AA release Moreover, that of LT the translocation of also and inhibited by a between inhibition of AA release and 5-LO translocation in human series of to the of AA in the inhibition of 5-LO translocation. in and the in inhibited 5-LO translocation and LT biosynthesis in and in of exogenous AA of LT biosynthesis and 5-LO translocation in the of a functional link between AA release and 5-LO translocation in activated human that were in 5-LO in by and to and to a and to that the effects of AA human in of AA to human a 5-LO translocation, and LT by and in that in agonist-activated and that the in AA release and LT biosynthesis in activated the the of AA in 5-LO translocation, the of the and inhibitor pyrrophenone LT biosynthesis and 5-LO translocation. that LT biosynthesis inhibited by pyrrophenone in and human 5-LO translocation completely in and and of pyrrophenone 5-LO translocation LT by the of AA of the inhibitor pyrrophenone 5-LO translocation in activated human human were pyrrophenone AA, were and the of 5-LO and 5-LO in and of that the in the that the MEK and U-0126 of and AA release in also that these MEK 5-LO translocation in human the of and U-0126 AA release and 5-LO translocation in activated that U-0126 AA release in agonist-activated in a were inhibition of AA release the inhibition of and 5-LO translocation and of AA to the the translocation 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to the to the of 5-LO and that AA, and translocation of 5-LO in pyrrophenone and that the of AA in 5-LO translocation the 5-LO in of the that the the of the that 5-LO to of the of 5-LO a to in and a and to 5-LO translocation in of the inhibition by of 5-LO translocation the by in the translocation of 5-LO in of by AA of 5-LO translocation to the in activated also a AA, and a functional between the and the of the 5-LO in these to a by LT biosynthesis in human

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.