291 SUPEROVULATORY RESPONSE IN BEEF CATTLE TREATED DURING THE FIRST FOLLICULAR WAVE FOLLOWING SYNCHRONIZATION OF OVULATION WITH A PROGESTIN DEVICE AND GnRH
Notice bibliographique
Résumé
Although we have previously shown that superstimulation during the first follicular wave resulted in a successful response (Carballo Guerrero D et al. 2007 Reprod. Fertil. Dev. 20, 226), the protocol required many interventions that could influence its application in the field. Therefore, two studies were designed to simplify the superstimulation treatment protocol. Experiment 1 was designed to determine whether it was necessary to remove the progesterone releasing device during the superstimulation protocol. Angus cows (n = 37) were superstimulated by two treatments in a crossover design. Cows in Group 1 (control) received a progesterone releasing device (Cue-Mate, Bioniche Animal Health, Belleville, ON, Canada) along with 0.150 mg D cloprostenol (PGF, Bioprost-D, Biotay, Argentina) IM, at random stages of the estrous cycle. Five days later, a second PGF was injected and Cue-Mates were removed, followed by GnRH (0.050 mg Lecirelina, Biosin-OV, Biotay) 36 h later; ovulation was expected to occur 30 to 36 h later. On Day 0 (36 h after GnRH) donors received a new Cue-Mate and superstimulation treatment was initiated with a total dose of 400 mg NIH-FSH-P1 of Folltropin-V (FSH, Bioniche Animal Health) in twice daily decreasing doses over 5 days. PGF was injected with the last two FSH injections and Cue-Mates were removed with the last FSH injection. Cows in Group 2 were treated similarly to those in the control group, except that Cue-mate devices were not replaced and remained in place for 13 days (i.e. Cue-mates were removed with the last FSH and PGF injection). All donors received 12.5 mg pLH (Lutropin-V, Bioniche Animal Health) 24 h after Cue-Mate removal and were AI 12 and 24 h later. Embryos were collected 7 days after pLH. Means were compared between groups by Student’s t-test. Superovulatory response and embryo production did not differ between groups. Mean (± SEM) number of ova/embryos collected and transferable embryos were 8.2 ± 1.0 and 4.1 ± 0.6 v. 9.8 ± 0.9 and 5.7 ± 0.7 for Groups 1 and 2, respectively (P > 0.2). Experiment 2 was designed to evaluate the effect of giving FSH for 4 v. 5 days. Simmental (n = 18) and Angus (n = 6) cows were superstimulated by the two treatment protocols in a crossover design. Cows in both groups were treated similarly to those in Group 2 in Experiment 1 (i.e. Cue-Mates were not replaced during treatment). Cows in Group 1 (control) received FSH over 5 days (as in Group 2 of Experiment 1); while those in Group 2 received the same dosage of FSH, but given in twice daily decreasing doses over 4 days (Cue-Mates were removed with the last FSH and PGF injections). Superovulatory response and embryo production did not differ among groups. Mean (± SEM) number of ova/embryos collected and transferable embryos were 13.5 ± 2.4 and 6.6 ± 1.1 v. 12.0 ± 1.9 and 5.8 ± 1.0 for Groups 1 and 2, respectively (P > 0.6). In conclusion, superstimulation of cattle at the time of emergence of the first follicular wave after ovulation results in an acceptable superovulatory response and all treatments evaluated were user-friendly and equally efficient.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».