Separation and quantitative recovery of mouse serum arylesterase and carboxylesterase activity

Paraoxonase-1 (PON1) is known to be associated with high density lipoproteins. We optimized buffer conditions to obtain quantitative recovery of PON1 (arylesterase) activity and analyzed the distribution of PON1 in mice using a combination of size-exclusion chromatography and ultracentrifugation. Size-exclusion chromatography of mouse serum separated the esterase activity into two peaks, one overlapping the high density lipoproteins and a second peak of lower molecular weight, consistent with serum carboxylesterase, which accounted for ∼20% of the total esterase activity of normal mouse serum. Using conditions for the quantitative recovery of arylesterase activity, we fractionated serum by ultracentrifugation into d < 1.21 g/ml, d < 1.25 g/ml, d > 1.21 g/ml, and d > 1.25 g/ml fractions. We observed PON1 arylesterase activity and in the d < 1.21 g/ml and serum in the d > 1.25 g/ml of the of PON1 arylesterase activity by serum by mice with a for of the in arylesterase activity in to is to the arylesterase activity mouse PON1 is associated with high density lipoproteins. of serum to the total esterase activity the of total arylesterase activity in mouse serum. Paraoxonase-1 (PON1) is known to be associated with high density lipoproteins. We optimized buffer conditions to obtain quantitative recovery of PON1 (arylesterase) activity and analyzed the distribution of PON1 in mice using a combination of size-exclusion chromatography and ultracentrifugation. Size-exclusion chromatography of mouse serum separated the esterase activity into two peaks, one overlapping the high density lipoproteins and a second peak of lower molecular weight, consistent with serum carboxylesterase, which accounted for ∼20% of the total esterase activity of normal mouse serum. Using conditions for the quantitative recovery of arylesterase activity, we fractionated serum by ultracentrifugation into d < 1.21 g/ml, d < 1.25 g/ml, d > 1.21 g/ml, and d > 1.25 g/ml fractions. We observed PON1 arylesterase activity and in the d < 1.21 g/ml and serum in the d > 1.25 g/ml of the of PON1 arylesterase activity by serum by mice with a for of the in arylesterase activity in to is to the arylesterase activity We mouse PON1 is associated with high density lipoproteins. of serum to the total esterase activity the of total arylesterase activity in mouse serum. Paraoxonase-1 (PON1) a of a in of in of by associated of high density associated of the activity of density of is of esterase activity and is by the is and be by by using by of PON1 is for the of PON1 to serum with by and the to is the of and by a high of of and mouse is in mice and is in the in with a in we observed a quantitative of PON1 arylesterase activity with mice serum separated by size-exclusion chromatography using a recovery of PON1 arylesterase activity serum ultracentrifugation to we observed a distribution of the arylesterase with the of normal mouse and high density distribution in mice serum esterase is in high in mouse serum in mouse is esterase and is the in mouse a PON1 arylesterase with a to the conditions in the of lipoproteins for with serum arylesterase and serum by ultracentrifugation of and 1.21 g/ml and by of in of by associated of high density associated of the activity of density of is of esterase activity and is by the for a of by the of by high density with a We the serum arylesterase is and buffer conditions for We mouse serum arylesterase activity and be by the and using mice and in the a the and for by the of mice a to of serum in a to the density to 1.21 1.25 g/ml, and the with d 1.21 g/ml d 1.25 g/ml and in a for and by the of to the the 1.21 g/ml to the density to 1.25 with d 1.25 g/ml and and chromatography of chromatography a the in PON1 activity associated with of be to a buffer and to serum fractions. of serum using a with a a using a a of with of with with a the and in a of and with of by of of in with of We combination of and in a with mouse serum the to the for in of and for in of for in of and in and with to and in of buffer of and and in a and for which buffer and for to and of arylesterase of serum to a total of in and the of the using a of the in for using a is of of with to of in of in and using a is of of using a and of optimized for mouse using total using and using using and using for mouse serum PON1 the of and mouse d < 1.21 g/ml serum separated by of in of and and to to for a using and and the peak of PON1 arylesterase activity the peak of by size-exclusion the distribution of arylesterase activity and serum in mice by chromatography We observed PON1 arylesterase activity the peak of by one PON1 in is to be activity and and mouse separated the of the in and the distribution of arylesterase activity using and the recovery of PON1 arylesterase a for by ultracentrifugation and of to the PON1 of serum serum the of and in the of and of the arylesterase activity, of in buffer to and the conditions of for the recovery of PON1 arylesterase We of to to PON1 activity and We the arylesterase activity of PON1 ultracentrifugation in of in for by PON1 arylesterase is associated with mouse is in of and a in and mouse of We serum mice and separated the serum by ultracentrifugation into d < 1.21 g/ml, d g/ml, and d > 1.25 g/ml fractions. PON1 arylesterase activity to the in the d < 1.21 g/ml and d < 1.25 g/ml PON1 arylesterase in the d > 1.21 g/ml d g/ml of mouse serum arylesterase activity recovery of and of arylesterase serum separated with a ultracentrifugation d 1.21 separated a and the analyzed for and distribution distribution of arylesterase activity observed with serum separated ultracentrifugation into the peak the d < 1.21 g/ml and into the peak the d > 1.21 g/ml lower for the d > 1.25 g/ml to observed for the d > 1.21 g/ml and recovery of and serum ultracentrifugation of mouse > > density of mouse serum to 1.21 g/ml with with 1.21 g/ml and and separated by ultracentrifugation into d g/ml and the d > 1.21 g/ml d > 1.21 g/ml to a density of 1.25 g/ml with and separated by ultracentrifugation into the d g/ml and the d > 1.25 g/ml density of a of serum to 1.25 g/ml and with 1.25 g/ml and two in a chromatography of of mouse serum. d < 1.21 g/ml and arylesterase activity d > 1.21 g/ml arylesterase activity and activity and esterase analyzed in and distribution of PON1 by of of the by We observed mouse serum with the in the molecular serum and d > 1.25 g/ml be analyzed of PON1 for the d < 1.21 g/ml separated by chromatography a with the peak of with the peak of arylesterase activity of PON1 with mouse PON1 activity the of of PON1 by be for the of a in the of to We to the and observed a distribution the of the arylesterase peak (PON1) and with the second esterase activity in the d > 1.25 g/ml of the activity of the d > 1.25 g/ml with of of PON1 and to the esterase the of the with and in the we to the to the esterase activity in the in which the two Using a the esterase of and to obtain a PON1 arylesterase of and arylesterase to and mouse is known to be to a in and Using mice to in a of and the the the serum arylesterase activity of to mice in of and be by in a the of to mice in of and be by mice the and serum and serum separated by chromatography and for and arylesterase activity using in and serum arylesterase activity by and by and by and by and and to and serum of the in and and in with the in and serum for to and to in the of a by chromatography of mouse serum. of mice in mice a mice the and and serum and and arylesterase analyzed in and of arylesterase activity and in serum of mice the and and arylesterase and and serum serum esterase activity by and and activity by the the activity by of esterase activity into two a the with esterase activity a with arylesterase activity a with with a high of the of the serum esterase activity and of the total serum esterase activity of total and arylesterase activity with observed the quantitative recovery of PON1 arylesterase activity, the distribution of activity in serum and in the d < 1.21 g/ml and distribution for activity and of mouse serum two associated with and in the d > 1.25 g/ml serum with of the arylesterase in the d g/ml of by the activity in to and in of and serum arylesterase activity and the of activity be by the of We the for the of PON1 arylesterase activity to arylesterase we in the of PON1 arylesterase of by activity, with high density lipoproteins (PON1) in and is in the the distribution of PON1 activity and for a of by of the PON1 activity and the of the activity associated with ultracentrifugation We the of activity to the of the in the of is the of of ultracentrifugation. We size-exclusion chromatography the for the distribution of Using and a ultracentrifugation ultracentrifugation to we the in the we the of PON1 in is separated by the peak for of a by a of with PON1 by consistent with PON1 in of of of the PON1 observed PON1 in the density 1.21 is associated with in observed associated with and PON1 a of observed quantitative of serum by chromatography accounted for a of the total activity, and associated with in and and mouse (PON1) in and of into mice with a serum mice and in the of PON1 in in serum is PON1 to the of and PON1 to a the of the and in the of the of the of PON1 in Paraoxonase-1 (PON1) a of a in of in of by associated of high density associated of the activity of density of is of esterase activity and is by the is and be by by using by of PON1 is for the of PON1 to serum with by and the to is the of and by a high of of and mouse is in mice and is in the in with a in we observed a quantitative of PON1 arylesterase activity with mice serum separated by size-exclusion chromatography using a recovery of PON1 arylesterase activity serum ultracentrifugation to we observed a distribution of the arylesterase with the of normal mouse and high density distribution in mice serum esterase is in high in mouse serum in mouse is esterase and is the in mouse a PON1 arylesterase with a to the conditions in the of lipoproteins for with serum arylesterase We and serum by ultracentrifugation of and 1.21 g/ml and by of in of by associated of high density associated of the activity of density of is of esterase activity and is by the for a of by the of by high density with a We the serum arylesterase is and buffer conditions for We mouse serum arylesterase activity and be by the and using mice and in the a the and for by the of mice a to of serum in a to the density to 1.21 1.25 g/ml, and the with d 1.21 g/ml d 1.25 g/ml and in a for and by the of to the the 1.21 g/ml to the density to 1.25 with d 1.25 g/ml and and chromatography of chromatography a the in PON1 activity associated with of be to a buffer and to serum fractions. of serum using a with a a using a a of with of with with a the and in a of and with of by of of in with of We combination of and in a with mouse serum the to the for in of and for in of for in of and in and with to and in of buffer of and and in a and for which buffer and for to and of arylesterase of serum to a total of in and the of the using a of the in for using a is of of with to of in of in and using a is of of using a and of optimized for mouse using total using and using using and using for mouse serum PON1 the of and mouse d < 1.21 g/ml serum separated by of in of and and to to for a using and and the mice and in the a the and for by the mice and in the a the and for by the of mice a to of serum in a to the density to 1.21 1.25 g/ml, and the with d 1.21 g/ml d 1.25 g/ml and in a for and by the of to the the 1.21 g/ml to the density to 1.25 with d 1.25 g/ml and and mice a to of serum in a to the density to 1.21 1.25 g/ml, and the with d 1.21 g/ml d 1.25 g/ml and in a for and by the of to the the 1.21 g/ml to the density to 1.25 with d 1.25 g/ml and and chromatography of chromatography a the in PON1 activity associated with of be to a buffer and to serum fractions. of serum using a with a a using a a of with of with with a the and in a of and with of by of of in with of We combination of and in a with mouse serum chromatography a the in PON1 activity associated with of be to a buffer and to serum fractions. of serum using a with a a using a a of with of with with a the and in a of and with of by of of in with of We combination of and in a with mouse serum the to the for in of and for in of for in of and in and with to and in of buffer of and and in a and for which buffer and for to and the to the for in of and for in of for in of and in and with to and in of buffer of and and in a and for which buffer and for to and of arylesterase of serum to a total of in and the of the using a of the in for using a is of of serum to a total of in and the of the using a of the in for using a is of of with to of in of in and using a is of with to of in of in and using a is of of using a and of optimized for mouse using total using and using using and using using a and of optimized for mouse using total using and using using and using for mouse serum PON1 the of and mouse d < 1.21 g/ml serum separated by of in of and and to to for a using and and the PON1 mouse serum PON1 the of and mouse d < 1.21 g/ml serum separated by of in of and and to to for a using and and the peak of PON1 arylesterase activity the peak of by size-exclusion the distribution of arylesterase activity and serum in mice by chromatography We observed PON1 arylesterase activity the peak of by one PON1 in is to be activity and and mouse separated the of the in and the distribution of arylesterase activity using and the recovery of PON1 arylesterase a for by ultracentrifugation and of to the PON1 of serum serum the of and in the of and of the arylesterase activity, of in buffer to and the conditions of for the recovery of PON1 arylesterase We of to to PON1 activity and We the arylesterase activity of PON1 ultracentrifugation in of in for by PON1 arylesterase is associated with mouse is in of and a in and mouse of We serum mice and separated the serum by ultracentrifugation into d < 1.21 g/ml, d g/ml, and d > 1.25 g/ml fractions. PON1 arylesterase activity to the in the d < 1.21 g/ml and d < 1.25 g/ml PON1 arylesterase in the d > 1.21 g/ml d g/ml of mouse serum arylesterase activity recovery of and of arylesterase serum separated with a ultracentrifugation d 1.21 separated a and the analyzed for and distribution distribution of arylesterase activity observed with serum separated ultracentrifugation into the peak the d < 1.21 g/ml and into the peak the d > 1.21 g/ml lower for the d > 1.25 g/ml to observed for the d > 1.21 g/ml and recovery of and serum ultracentrifugation of mouse > > density of mouse serum to 1.21 g/ml with with 1.21 g/ml and and separated by ultracentrifugation into d g/ml and the d > 1.21 g/ml d > 1.21 g/ml to a density of 1.25 g/ml with and separated by ultracentrifugation into the d g/ml and the d > 1.25 g/ml density of a of serum to 1.25 g/ml and with 1.25 g/ml and two in a distribution of PON1 by of of the by We observed mouse serum with the in the molecular serum and d > 1.25 g/ml be analyzed of PON1 for the d < 1.21 g/ml separated by chromatography a with the peak of with the peak of arylesterase activity of PON1 with mouse PON1 activity the of of PON1 by be for the of a in the of to We to the and observed a distribution the of the arylesterase peak (PON1) and with the second esterase activity in the d > 1.25 g/ml of the activity of the d > 1.25 g/ml with of of PON1 and to the esterase the of the with and in the we to the to the esterase activity in the in which the two Using a the esterase of and to obtain a PON1 arylesterase of and arylesterase to and mouse is known to be to a in and Using mice to in a of and the the the serum arylesterase activity of to mice in of and be by in a the of to mice in of and be by mice the and serum and serum separated by chromatography and for and arylesterase activity using in and serum arylesterase activity by and by and by and by and and to and serum of the in and and in with the in and serum for to and to in the of a by chromatography of mouse serum. of mice in mice a mice the and and serum and and arylesterase analyzed in and of arylesterase activity and in serum of mice the and and arylesterase and and serum serum esterase activity by and and activity by the the activity by of esterase activity into two a the with esterase activity a with arylesterase activity a with with a high of the of the serum esterase activity and of the total serum esterase activity of total and arylesterase activity with peak of PON1 arylesterase activity the peak of by size-exclusion the distribution of arylesterase activity and serum in mice by chromatography We observed PON1 arylesterase activity the peak of by one PON1 in is to be activity and and mouse separated the of the in and the distribution of arylesterase activity using ultracentrifugation. We the distribution of arylesterase activity and serum in mice by chromatography We observed PON1 arylesterase activity the peak of by one PON1 in is to be activity and and mouse separated the of the in and the distribution of arylesterase activity using ultracentrifugation. and the recovery of PON1 arylesterase a for by ultracentrifugation and of to the PON1 of serum serum the of and in the of and of the arylesterase activity, of in buffer to and the conditions of for the recovery of PON1 arylesterase We of to to PON1 activity and We the arylesterase activity of PON1 ultracentrifugation in of in for by We a for by ultracentrifugation and of to the PON1 of serum serum the of and in the of and of the arylesterase activity, of in buffer to and the conditions of for the recovery of PON1 arylesterase We of to to PON1 activity and We the arylesterase activity of PON1 ultracentrifugation in of in for by PON1 arylesterase is associated with mouse is in of and a in and mouse of We serum mice and separated the serum by ultracentrifugation into d < 1.21 g/ml, d g/ml, and d > 1.25 g/ml fractions. PON1 arylesterase activity to the in the d < 1.21 g/ml and d < 1.25 g/ml PON1 arylesterase in the d > 1.21 g/ml d g/ml of mouse serum arylesterase activity recovery of and of arylesterase serum separated with a ultracentrifugation d 1.21 separated a and the analyzed for and distribution distribution of arylesterase activity observed with serum separated ultracentrifugation into the peak the d < 1.21 g/ml and into the peak the d > 1.21 g/ml lower for the d > 1.25 g/ml to observed for the d > 1.21 g/ml and recovery of and serum ultracentrifugation of mouse > > density of mouse serum to 1.21 g/ml with with 1.21 g/ml and and separated by ultracentrifugation into d g/ml and the d > 1.21 g/ml d > 1.21 g/ml to a density of 1.25 g/ml with and separated by ultracentrifugation into the d g/ml and the d > 1.25 g/ml density of a of serum to 1.25 g/ml and with 1.25 g/ml and two in a distribution of PON1 by of of the by We observed mouse serum with the in the molecular serum and d > 1.25 g/ml be analyzed of PON1 for the d < 1.21 g/ml separated by chromatography a with the peak of with the peak of arylesterase activity of PON1 with mouse PON1 activity the of of PON1 by be for the of a in the of to We to the and observed a distribution the of the arylesterase peak (PON1) and with the second esterase activity in the d > 1.25 g/ml of the activity of the d > 1.25 g/ml with of of PON1 and to the esterase the of the with and in the we to the to the esterase activity in the in which the two Using a the esterase of and to obtain a PON1 arylesterase mouse is in of and a in and mouse of We serum mice and separated the serum by ultracentrifugation into d < 1.21 g/ml, d g/ml, and d > 1.25 g/ml fractions. PON1 arylesterase activity to the in the d < 1.21 g/ml and d < 1.25 g/ml PON1 arylesterase in the d > 1.21 g/ml d g/ml of mouse serum arylesterase activity recovery of and of arylesterase serum separated with a ultracentrifugation d 1.21 separated a and the analyzed for and distribution distribution of arylesterase activity observed with serum separated ultracentrifugation into the peak the d < 1.21 g/ml and into the peak the d > 1.21 g/ml lower for the d > 1.25 g/ml to observed for the d > 1.21 g/ml density of mouse serum to 1.21 g/ml with with 1.21 g/ml and and separated by ultracentrifugation into d g/ml and the d > 1.21 g/ml d > 1.21 g/ml to a density of 1.25 g/ml with and separated by ultracentrifugation into the d g/ml and the d > 1.25 g/ml density of a of serum to 1.25 g/ml and with 1.25 g/ml and two distribution of PON1 by of of the by We observed mouse serum with the in the molecular serum and d > 1.25 g/ml be analyzed of PON1 for the d < 1.21 g/ml separated by chromatography a with the peak of with the peak of arylesterase activity of PON1 with mouse PON1 activity the of of PON1 by be for the of a in the of to We to the and observed a distribution the of the arylesterase peak (PON1) and with the second esterase activity in the d > 1.25 g/ml of the activity of the d > 1.25 g/ml with of of PON1 and to the esterase the of the with and in the we to the to the esterase activity in the in which the two Using a the esterase of and to obtain a PON1 arylesterase of and arylesterase to and mouse is known to be to a in and Using mice to in a of and the the the serum arylesterase activity of to mice in of and be by in a the of to mice in of and be by mice the and serum and serum separated by chromatography and for and arylesterase activity using in and serum arylesterase activity by and by and by and by and and to and serum of the in and and in with the in and serum for to and to in the of arylesterase activity and in serum of mice the and and arylesterase and and serum serum esterase activity by and and activity by the the activity by of esterase activity into two a the with esterase activity a with arylesterase activity a with with a high of the of the serum esterase activity and of the total serum esterase activity of total and arylesterase activity with mouse is known to be to a in and Using mice to in a of and the the the serum arylesterase activity of to mice in of and be by in a the of to mice in of and be by mice the and serum and serum separated by chromatography and for and arylesterase activity using in and serum arylesterase activity by and by and by and by and and to and serum of the in and and in with the in and serum for to and to in the serum esterase activity by and and activity by the the activity by of esterase activity into two a the with esterase activity a with arylesterase activity a with with a high of the of the serum esterase activity and of the total serum esterase activity observed the quantitative recovery of PON1 arylesterase activity, the distribution of activity in serum and in the d < 1.21 g/ml and distribution for activity and of mouse serum two associated with and in the d > 1.25 g/ml serum with of the arylesterase in the d g/ml of by the activity in to and in of and serum arylesterase activity and the of activity be by the of We the for the of PON1 arylesterase activity to arylesterase we in the of PON1 arylesterase of by activity, with high density lipoproteins (PON1) in and is in the the distribution of PON1 activity and for a of by of the PON1 activity and the of the activity associated with ultracentrifugation We the of activity to the of the in the of is the of of ultracentrifugation. We size-exclusion chromatography the for the distribution of Using and a ultracentrifugation ultracentrifugation to we the in the we the of PON1 in is separated by the peak for of a by a of with PON1 by consistent with PON1 in of of of the PON1 observed PON1 in the density 1.21 is associated with in observed associated with and PON1 a of observed quantitative of serum by chromatography accounted for a of the total activity, and associated with in and and mouse (PON1) in and of into mice with a serum mice and in the of PON1 in in serum is PON1 to the of and PON1 to a the of the and in the of the of the of PON1 in We observed the quantitative recovery of PON1 arylesterase activity, the distribution of activity in serum and in the d < 1.21 g/ml and distribution for activity and of mouse serum two associated with and in the d > 1.25 g/ml serum with of the arylesterase in the d g/ml of by the activity in to and in of and serum arylesterase activity and the of activity be by the of We the for the of PON1 arylesterase activity to arylesterase we in the of PON1 arylesterase of by activity, with high density lipoproteins (PON1) in and is in the the distribution of PON1 activity and for a of by of the PON1 activity and the of the activity associated with ultracentrifugation We the of activity to the of the in the of is the of of ultracentrifugation. We size-exclusion chromatography the for the distribution of Using and a ultracentrifugation ultracentrifugation to we the in the we the of PON1 in is separated by the peak for of a by a of with PON1 by consistent with PON1 in of of of the PON1 observed PON1 in the density 1.21 is associated with in observed associated with and PON1 a of observed quantitative of serum by chromatography accounted for a of the total PON1 activity, and associated with in and and mouse (PON1) in and of into mice with a serum mice and in the of PON1 in in serum is PON1 to the of and PON1 to a the of the and in the of the of the of PON1 in by a to the and of and in by to serum chromatography

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.