93 SEX-SPECIFIC DEVELOPMENTAL PROGRAMMING OF THE BOVINE EMBRYO BY COLONY STIMULATING FACTOR 2 (CSF2)
Notice bibliographique
Résumé
Competence of the bovine embryo to establish pregnancy can be enhanced by treatment with colony stimulating factor 2 (CSF2) from Days 5 to 7 post-insemination. The mechanism is unknown but could involve developmental reprogramming. Here, we questioned whether treatment with CSF2 from Days 5 to 7 alters growth, interferon-t (IFNT) secretion, and the methylome of extra-embryonic membranes (EEM) at Day 15. In vitro-produced embryos were treated with either 0 or 10 ng mL–1 bovine CSF2 from Days 5 to 7. Expanded blastocysts were transferred into synchronized recipients. On Day 15, conceptuses were recovered by flushing the uterus and sex determined by PCR (n = 4 males and 4 females per treatment). Statistical analysis of IFNT and length was performed using analysis of variance with the general linear models procedure of SAS (SAS Institute Inc., Cary, NC, USA). There was a sex × treatment interaction for conceptus length (P < 0.003) and IFNT in uterine flushing (P < 0.05) (as determined by antiviral activity). CSF2 increased length in males (least squares means ± s.e.m.: 93 ± 19 mm v. 20 ± 19 mm) and decreased length in females (22 ± 19 mm v. 103 ± 19). Similarly, CSF2 increased IFNT in males (45 483 ± 22 615 IU mL–1v. –2536.27 ± 13 385 IU mL–1) and decreased IFNT in females (–14 012 ± 13 642 IU mL–1v. 35 404 ± 7514.91 IU mL–1). The EmbryoGENE DNA Methylation Array (Edmonton, AB, Canada) was used to assess CSF2 effects at 418 805 positions across the genome in a subset of EEM (n = 2 for vehicle and 4 for CSF2 in males; n = 3 for vehicle and n = 3 for CSF2 in females). CSF2 caused hypermethylation for 9842 probes in males and 6227 probes in females and hypomethylation for 9322 probes in males and 3292 probes in females. An analysis was conducted to evaluate if clusters of differentially-methylated probes were non-randomly distributed spatially in 5 Mb regions of individual chromosomes using a 500-kb moving window. Statistical significance was determined using chi-squared tests for each 500-kb window. Differential methylation was not uniformly distributed but rather there were regions or hyper- and hypomethylation that varied with sex. The most common situation was where a region was differentially methylated in one sex but not the other. In some cases, a region was differentially methylated in opposite directions for males and females. For example, a region of BTA1 between positions 67 Mb–77.5 Mb contained 1632 probes, of which 59 probes were hypo-methylated in males and 35 were hypermethylated in females. In conclusion, changes in developmental programming of the bovine embryo caused by CSF2 occur in a sex-specific manner. This result suggests a possible mechanism by which environmental effects on the female affect male embryos differently than female embryos. Support was provided by U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) grant 2011-67015-30688.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».