Impact of New Natural Biostimulants on Increasing Synthesis in Plant Cells of Small Regulatory si/miRNA With High Anti-Nematodic Activity
Notice bibliographique
Résumé
Plant endoparasitic cyst nematode Heterodera schachtii Schmidt, gallic nematode Meloidogyne incognita and stem nematode Ditylenchus destructor damage various agricultural crops. The application of ecologically safe natural biostimulants with bioprotective properties is a newer approach for increasing plant resistance to parasitic nematodes. The molecular-genetic analysis of biostimulants action on plant genome is necessary for creation of new effective bioregulators for plant protection against phytopathogenic organisms. In our field and greenhouse experiments, we investigated the influence of new natural biostimulants Avercom and its derivatives on plant protection against nematodes Meloidogyne incognita and Ditylenchus destructor. Considerable increase of resistance to nematodes and productivity of cucumber and potato were observed for plants treated by biostimulant Avercom and its derivatives. Impact of biostimulants Radostim-super and Avercom on increase of resistance of sugar beet and cucumber sprouts to nematodes Heterodera schachtii and Meloidogyne incognita was studied in the laboratory conditions. Comparative analysis of morpho-physiological signs of control and experimental plants showed that plants treated by Radostim-super and Avercom were more viable and resistant to these nematodes as compared to control sprouts. In the molecular-genetic experiments, we studied the impact of these biostimulants on inducing synthesis of small regulatory si/miRNA, which plays key role in plant immune protection. Using method Dot-blot hybridization we studied degree of homology between si/miRNA with mRNA populations, isolated from plants untreated and treated with new natural biostimulants. We found considerable difference in the degree of homology (6-28%) between populations of mRNA and si/miRNA from nematode-infected plants that were either untreated or treated with biostimulants. We have also investigated silencing of translation of mRNA activity of si/miRNA in the wheat embryo cell-free system of protein synthesis. In these experiments, we found high inhibitory activity (38-65%) of si/miRNA from plants treated by biostimulants as compared to low inhibitory activity (15-20%) of si/miRNA from untreated plants. Obtained differences in the degree of homology between populations of mRNA and si/miRNA from untreated and treated with biostimulants plants, which were infected by nematode, and also the high inhibitory activity of si/miRNA from plants treated by biostimulants confirm that these biostimulants induce synthesis of anti-nematodic si/miRNA in plants, resulting in considerable increase of their resistance to these phytopathogens.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».