Factors affecting hepatocyte isolation, engraftment, and replication in an in vivo model
Notice bibliographique
Résumé
We congratulate Kawahara et al.1 on their excellent study published in Liver Transplantation.We read about their experiences with human hepatocyte isolation and the subsequent application of the isolated cells with keen interest. Our group also has good experience with human hepatocyte isolation.2 We have 2 points to raise with respect to this excellent study. The authors used human hepatocytes isolated from resected normal liver tissue in their study (51 cancer specimens and 39 specimens of benign etiology). Our data show that such hepatocytes differ in their response to hypoxia and hypoxia/reoxygenation in comparison with hepatocytes isolated from normal livers.2 Did the authors note any phenotypic differences between hepatocytes isolated from cancer specimens and those isolated from benign livers? Moreover, did this have a bearing on transplant success? Kawahara et al.1 stated that their initial experiences with the isolation of human hepatocytes from cirrhotic livers was poor in terms of viable cell yields and transplant success. In our laboratory, we isolate human hepatocytes from all explanted livers after transplantation (for primary biliary cirrhosis, primary sclerosing cholangitis, alcoholic liver cirrhosis, or metabolic liver disease), from donor livers exceeding surgical requirements, and from normal tissue obtained from hepatic resections (for metastatic disease). We do not isolate the hepatocytes of patients with a viral hepatic disease. Although we have not published our results yet, we can state that our overall success rate for attempted human hepatocyte isolation is 54%. Because we use all liver tissue that is available to us, we have viable cell yields of 20,000 to 5,000,000 cells/mL. Therefore, we suggest that cirrhotic livers could be a valuable source of human hepatocytes for experimental use. We concur with Kawahara et al. that human hepatocytes isolated from extended criteria donors are suitable for cell transplantation, but we also stress that human hepatocytes isolated from diseased livers can be a valuable source of cells for functional studies.2 We again congratulate the authors on their excellent work within this challenging area, and we hope that with continuing research in human hepatocyte isolation, clear parameters and protocols can be established so that hepatocytes can be harvested from all available livers. Ricky H. Bhogal M.R.C.S.Ed [email protected]*, Simon C. Afford Ph.D., FRCPath*, * Centre for Liver Research Institute for Biomedical Research University of Birmingham Birmingham, United Kingdom.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,002 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».