A surgical technique for administering laser irradiation to rat incisor dentin
Notice bibliographique
Résumé
With the advent of lasers in the field of dentistry, various methods have been used to expose dentin to laser irradiation. This was done indirectly through the alveolar bone, as well as directly on dentin wafers and on extracted human teeth. However, dentin is a vital tissue and the only way to show the ramifications of laser irradiation is by directly exposing the dentin to this energy source under in vivo conditions. In the present study we describe a highly reproducible method for accomplishing this. Male Sprague Dawley rats were anesthetized and an incision was made at the junction of the attached palatal mucosa and the unattached buccal mucosa parallel to the maxillary bones on both sides. This procedure exposed the underlying bone where a 2 mm square window, at a point 3 mm caudal and parallel to the gingival attachment, was cut out to the depth of the periodontal ligament. The site was flushed with a 0.9% sterile sodium chloride solution and gently dried with gauze. The beam of the laser was focused in the middle of the 2 mm square window and a pre-determined energy level and pulse duration (2 W 50 ms or 10 W 50 ms) were selected. The focal length guide was held at right angles to the dentin surface and in contact with the maxilla at all times. The energy densities of either 52.6 or 263.2 J/cm(2) were delivered to the 'root' dentin by a single pulse. The survival rate was 92%. The animals that died did so while under anesthesia and not after exposure to laser irradiation. The animals that survived tolerated the surgery and the laser irradition very well, regaining (and eventually surpassing) their initial weight 3 days after the procedures were performed. The thickness of the dentin that was exposed to laser irradiation was not completely penetrated by the energy levels used. At both energy levels some dentinal tubules appeared blocked and evidence of melted dentin was apparent. However, at the higher energy level two distinct zones were observed in the funnel-shaped lesion. In the inner zone distinct dentinal tubules were not readily visible, while in the outer zone the tubules were opened and completely free of debris. This technique is highly reproducible and yields consistent results with little or no stress to the animals. Although the energy levels used did not penetrate the entire thickness of the dentin, the depth of penetration of the laser beam appears to be related to the energy level used and the thickness of the dentin layer present.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».