371 INTRACYTOPLASMIC SPERM INJECTION OF EQUINE OOCYTES USING AIR-DRIED SPERM OR SPERM STORED IN A HIGH OSMOLARITY MEDIUM
Notice bibliographique
Résumé
The generally accepted method of long-term sperm preservation is freezing in liquid nitrogen. However, it is not always available. Other techniques have shown to preserve sperm for a short period of time that can be used for intracytoplasmic sperm injection (ICSI). Mouse offspring have been produced after ICSI with sperm stored in a high osmolarity medium. Also, human embryos were obtained by ICSI with air-dried sperm. Recently, equine blastocysts have been obtained by ICSI with lyophilized sperm. In our study, cleavage rate was evaluated after ICSI of equine oocytes using air-dried sperm or sperm stored in a high osmolarity medium. Oocytes were obtained from slaughterhouse ovaries by scraping individual follicles and transported in a portable incubator at 38°C for 15 h in TCM-199 buffered with HEPES and supplemented with glutamine, sodium pyruvate, LH (Bioniche Animal Health, Inc., Beltville, Ontario, Canada), FSH (Bioniche Animal Health, Inc.), epidermal growth factor (EGF), insulin-like growth factor 1 (IGF-1), and 10% FBS (GIBCO, Grand Island, NY, USA). On arrival, they were cultured for 6 more hours in 5% CO2 in air at 38°C in microdroplets of the same medium, without HEPES. Sperm was collected from 2 stallions of proven fertility. The ejaculate was diluted in Kenney extender, centrifuged, and the pellet was resuspended in HEPES-TALP (H-TALP). Three sperm treatments were used: (C) Control: ejaculated motile sperm processed as described above; (1) Air-dried sperm: obtained from spreading sperm on a sterile slide and drying it for 10 min in a laminar flow chamber; (2) Sperm in a high osmolarity medium: ejaculated motile sperm resuspended in H-TALP with high osmolarity (800 mOsmol). Samples from groups 1 and 2 were stored at 5°C for 2 to 3 days before being used for sperm injection. Only oocytes with an intact cytoplasm and a visible polar body were selected for injection and randomly assigned to each experimental group. Each MII oocyte was injected with 1 sperm cell and activated in Ionomicin for 10 min and DMAP for 3 h. Injected oocytes were cultured in DMEM : F10 1 : 1 with 10% FBS at 38°C in 7% O2 and 5% CO2 for 48 h. The number of cleaved embryos was recorded. Data was analyzed by chi-square test. A total of 135 MII oocytes were injected. The cleavage rate in group 1 was significantly lower than in the control group (31/71, 43.66% vs. 28/38, 73.68%) ( P < 0.05). No differences were observed between group (2) and control (19/29, 73.07% vs. 28/38, 73.68%) ( P > 0.05). This is the first report of equine oocytes fertilized by ICSI with air-dried sperm or with sperm kept in high osmolarity medium. These simple sperm preservation techniques might be an alternative option when liquid nitrogen is not available. Further studies will determine if it is possible to obtain pregnancies or even healthy offspring.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,005 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».