Comparison of the regenerative capacity of the cerebral cortex in neonatal mice following traumatic or ischemic injury
Notice bibliographique
Résumé
We have previously shown that the murine medial frontal cortex (MFC) regenerates, anatomically and functionally, when it is removed by aspiration during the early postnatal period. The cells that repopulate the aspirated MFC arise from progenitor cells in the lateral ventricles that migrate into the lesion site rather than to their normal target, the olfactory bulbs. The possibility that the postnatal brain is capable of large-scale neuronal replacement has obvious implications for pathological conditions that result in large areas of cell death, however, this has not been directly addressed. In the present study we assessed whether the neonatal MFC was equally capable of regeneration when it is damaged using a clinically relevant model of injury - stroke. We also set out to identify the molecular factors that affect regeneration following brain injury. We adopted a model of stroke for use in the neonatal mouse that is relatively non-invasive and very reliable in producing an area of injury identical to that provided by the aspiration method. In this model, the photoreactive dye, rose bengal, was injected systemically (50 mg/kg; i.p) on postnatal day (PD) 7 and the MFC was irradiated through the intact skull with laser light (532 nm; 20 mW; 90 s). This reaction causes platelets to aggregate and a thrombus to form in the laser-exposed blood vessels. The regenerative capacity of the MFC in these animals was compared to that of animals experiencing aspiration lesions on PD7. All animals were administered BrdU (50 mg/kg once daily for 2 or 3 days post-lesion) and were allowed to survive from 2 to 8 days post-lesion, after which the brains were removed for anatomical evaluation. The number and phenotypes of cells occupying the aspiration and ischemic lesion area were compared using BrdU, and neuron- and glia-specific antibodies. A proteomics approach was used to investigate the molecular factores affecting regeneration, where we compared the proteins expressed in and around the lesion area from both modes of injury using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). We found that, unlike aspiration lesions, regeneration of the MFC did not occur following damage induced by focal ischemia. Using 2D-PAGE we were able to resolve and compare the expression of over 1200 proteins in the lesion area; 174 that were unique to aspiration-injury, and 100 that were unique to ischemic injury. The characterization of these proteins will allow us to identify those factors that facilitate or prevent regeneration following injury induced in the neonatal brain.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,001 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,001 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».