Suppression of NF-κB Activation by Entamoeba histolytica in Intestinal Epithelial Cells Is Mediated by Heat Shock Protein 27

Little is known about the pathogenesis of Entamoeba histolytica and how epithelial cells respond to the parasite. Herein, we characterized the interactions between E. histolytica and colonic epithelial cells and the role macrophages play in modulating epithelial cell responses. The human colonic epithelial cell lines Caco-2 and T84 were grown either as monoculture or co-cultured in transwell plates with differentiated human THP-1 macrophages for 24 h before stimulation with soluble amebic proteins (SAP). In naive epithelial cells, prolonged stimulation with SAP reduced the levels of heat shock protein (Hsp) 27 and 72. However in THP-1 conditioned intestinal epithelial cells SAP enhanced Hsp27 and Hsp72, which was dependent the of SAP and with SAP the of and of were the role of Hsp27 in we Hsp27 with and and was in conditioned epithelial of Hsp27 the of of Hsp27 In conditioned epithelial cells, Hsp27 was prolonged to the of colonic in the of with E. Little is known about the pathogenesis of Entamoeba histolytica and how epithelial cells respond to the parasite. Herein, we characterized the interactions between E. histolytica and colonic epithelial cells and the role macrophages play in modulating epithelial cell responses. The human colonic epithelial cell lines Caco-2 and T84 were grown either as monoculture or co-cultured in transwell plates with differentiated human THP-1 macrophages for 24 h before stimulation with soluble amebic proteins (SAP). In naive epithelial cells, prolonged stimulation with SAP reduced the levels of heat shock protein (Hsp) 27 and 72. However in THP-1 conditioned intestinal epithelial cells SAP enhanced Hsp27 and Hsp72, which was dependent the of SAP and with SAP the of and of were the role of Hsp27 in we Hsp27 with and and was in conditioned epithelial of Hsp27 the of of Hsp27 In conditioned epithelial cells, Hsp27 was prolonged to the of colonic in the of with E. The intestinal Entamoeba histolytica The is characterized and of the role of and in colonic the to amebic is the of to the of amebic E. epithelial cells and role of E. and as soluble amebic heat shock intestinal epithelial heat shock soluble amebic heat shock intestinal epithelial heat shock as for is of E. of intestinal and of the the of of amebic cells the of and to the cells of in to cells in respond in in with and cells in the in were to the epithelial cell in the of cells as and was in epithelial cell lines to and the of with macrophages epithelial cell to the of of of proteins heat shock proteins (Hsp) and is to as heat shock or and to in cell intestinal epithelial cells to the cells as or cell of in to as E. and as and epithelial is and cells as is the to and of cells to epithelial cell and macrophages of we the of macrophages epithelial cell E. is in the of we to the in and colonic epithelial cells and and the we epithelial cells for in to amebic Hsp27 as the of with in intestinal epithelial cells of the which colonic is in the of E. and the of in to the with amebic and colonic cell lines T84 and Caco-2 were and in and with and THP-1 macrophages were and in with in plates were to in the of for The macrophages were and in for 24 h to amebic proteins were of of E. histolytica and the E. histolytica were as and and and of the human Hsp72, and Hsp27 were Hsp27 and was was The and were to of with or Caco-2 cells between and grown in either or transwell plates for to were Caco-2 cells grown for were cell THP-1 was differentiated with for and for 24 h before with epithelial cells were in plates macrophages for 24 h for and for cells were and and for amebic epithelial cells were the and and for and were in and was The were in with in with and with in in and the was with the enhanced to the were the and protein heat shock to of the human or was with were The of of of of of in the of and and for were in The were and with was in and to cells h of the was and the cells were with the was the cells for in the of of the and the the cell were and the were were in with with of cell were with of was with in and to and cells were with Hsp27 or as the Caco-2 cells were for h the of The cells were for SAP and of and Hsp27 in and in which and were Caco-2 cells were with and to the of was protein The cells were for 24 h co-cultured with differentiated and the of 24 conditioned was with SAP and as were with cell and with the of of were with and with and were in of and for of was as The was with for the protein was the was and the were The cell were for for were and the were the to was with The were to and the in of the the of Hsp27 and in and the of is and to we the of SAP Hsp27 and in the were in Caco-2 and T84 colonic epithelial cells levels of and in and conditioned and SAP SAP was to as as and was for the of with 24 h was to epithelial cells for the for epithelial cell to for Caco-2 cells were with 24 h for h before with SAP for and for play in and and 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of to was to the of colonic in amebic E. and is to we the of amebic protein we with SAP for h and with with amebic proteins of and in colonic epithelial proteins were with SAP or for h before with of for of was to as The cells were with with or with and was and to the The with The was cells with The were and with for of cell the of with or SAP and stimulation or with was with SAP or for h before with for In was with as The as The is of cell to of of the of to is we in in in amebic was was is in to which we Hsp27 and and the in Hsp27 in the of Hsp27 and amebic protein of with Hsp27 or were with SAP for h of stimulation with was as and for were with either or for h as and the cell were with with in and known to with and in cell lines for the we the between and in intestinal epithelial cells and and with and with SAP the of Hsp27 with of Hsp27 reduced and Hsp27 of the and with in Caco-2 and Hsp27 with was enhanced SAP with in intestinal epithelial of with Caco-2 cell were with and with or 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intestinal epithelial cells to the cells as or cell of in to as E. and as and epithelial is and cells as is the to and of cells to epithelial cell and macrophages of we the of macrophages epithelial cell E. is in the of we to the in and colonic epithelial cells and and the we epithelial cells for in to amebic Hsp27 as the of with in intestinal epithelial cells of the which colonic is in the of E. and the of in to the with amebic and colonic cell lines T84 and Caco-2 were and in and with and THP-1 macrophages were and in with in plates were to in the of for The macrophages were and in for 24 h to amebic proteins were of of E. histolytica and the E. histolytica were as and and and of the human Hsp72, and Hsp27 were Hsp27 and was was The and were to of with or Caco-2 cells between and grown in either or transwell plates for to were Caco-2 cells grown for were cell THP-1 was differentiated with for and for 24 h before with epithelial cells were in plates macrophages for 24 h for and for 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and colonic cell lines T84 and Caco-2 were and in and with and THP-1 macrophages were and in with in plates were to in the of for The macrophages were and in for 24 h to amebic proteins were of of E. histolytica and the E. histolytica were as and and and of the human Hsp72, and Hsp27 were Hsp27 and was was The and were to of with or Caco-2 cells between and grown in either or transwell plates for to were Caco-2 cells grown for were cell THP-1 was differentiated with for and for 24 h before with epithelial cells were in plates macrophages for 24 h for and for cells were and and for amebic epithelial cells were the and and for and were in and was The were in with in with and with in in and the was with the enhanced to the were the and protein heat shock to of the human or was with were The of of of of of in the of and and for were in The were and with was in and to cells h of the was and the cells were with the was the cells for in the of of the and the the cell were and the were were in 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Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.