396 PRESENCE OF GRP78 ON THE CELL SURFACE OF KIDNEY CANCER CELLS CORRELATES WITH INCREASED ANTI-GRP78 AUTOANTIBODY TITRES IN KIDNEY CANCER PATIENTS- A POTENTIAL NOVEL DIAGNOSTIC MARKER
Notice bibliographique
Résumé
You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011396 PRESENCE OF GRP78 ON THE CELL SURFACE OF KIDNEY CANCER CELLS CORRELATES WITH INCREASED ANTI-GRP78 AUTOANTIBODY TITRES IN KIDNEY CANCER PATIENTS- A POTENTIAL NOVEL DIAGNOSTIC MARKER Richard Austin, Mario Gonzalez-Gronow, Sarka Lhotak, Ali Al-Hashimi, Liz Crane, Anil Kapoor, and Jehonathan Pinthus Richard AustinRichard Austin Hamilton, Canada More articles by this author , Mario Gonzalez-GronowMario Gonzalez-Gronow Durham, NC More articles by this author , Sarka LhotakSarka Lhotak Hamilton, Canada More articles by this author , Ali Al-HashimiAli Al-Hashimi Hamilton, Canada More articles by this author , Liz CraneLiz Crane Hamilton, Canada More articles by this author , Anil KapoorAnil Kapoor Hamilton, Canada More articles by this author , and Jehonathan PinthusJehonathan Pinthus Hamilton, Canada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.484AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES GRP78 is an endoplasmic reticulum (ER)-resident molecular chaperone that functions in correct folding of newly synthesized proteins. Translocation of GRP78 to the cell surface allows it to function as a unique signaling receptor to promote cell proliferation and survival. Furthermore, exposure of GRP78 on the surface of cancer cells stimulates the production of anti-GRP78 autoantibodies which correlate with accelerated cancer progression, enhanced metastatic potential and reduced survival. The purpose of these study was to assess the expression of cell surface GRP78 in kidney cancer specimens as well as the presence of anti-GRP78 autoantibodies in the serum of patients with kidney cancer. METHODS Tumor and normal surrounding renal parenchyma as well as serum samples were obtained prospectively from 65 with kidney cancer patients who underwent partial or radical nephrectomy in the past year (2009) in our institution. Immunocytochemistry staining with anti-GRP78 antibodies was performed on all specimens. Anti-GRP78 autoantibody titres in the serum were determined using ELISA assay and compared to that of 14 control non-kidney cancer patients. Changes in the activity of tissue factor procoagulant following treatment of CRL-1932 human clear cell renal cancer cells with purified anti-GRP78 autoantibodies were measured. RESULTS Prominent GRP78 immunostaining was observed on the cell surface of RCC biopsies. Normal perinuclear immunostaining for GRP78 was observed in adjacent cells from normal renal tissue. Anti-GRP78 autoantibody titres were significantly elevated (p<0.01) from the blood of RCC patients, compared to age-matched controls. The addition of purified anti-GRP78 autoantibodies to cultured human RCC cells caused a significant elevation in tissue factor procoagulant activity, an established indicator of cell surface GRP78 activation. CONCLUSIONS Our findings demonstrate the presence of GRP78 on the surface of RCC cells and correlates with an increase in anti-GRP78 autoantibody titres in blood of kidney cancer patients but not in healthy controls. The diagnostic and prognostic potential of serum anti-GRP78 autoantibodies should be further explored. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185 Issue 4S April 2011 Page: e160 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.Metrics Author Information Richard Austin Hamilton, Canada More articles by this author Mario Gonzalez-Gronow Durham, NC More articles by this author Sarka Lhotak Hamilton, Canada More articles by this author Ali Al-Hashimi Hamilton, Canada More articles by this author Liz Crane Hamilton, Canada More articles by this author Anil Kapoor Hamilton, Canada More articles by this author Jehonathan Pinthus Hamilton, Canada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,021 | 0,005 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».