Abstract 3277: The Fps/Fes protein-tyrosine kinase serves a stromal tissue-specific supporting role in an orthotopic mouse model of breast cancer
Notice bibliographique
Résumé
Abstract Fes (also known as Fps) is a cytoplasmic protein tyrosine kinase that is highly expressed in breast epithelial cells during lactation, as well as in vascular endothelial and myeloid cells. Retrovirally encoded oncogenic alleles of Fps/Fes are associated with tumors in chickens and cats and cause tumors in transgenic mice; however, a role for Fes in human cancer has not been established. We hypothesized that Fes promotes breast tumorigenesis through tumor cell intrinsic functions as well as through functions in stromal tissues including vascular endothelial cells and macrophages. To test the potential tumor cell intrinsic role, AC2M2 cells, a highly metastatic mouse mammary carcinoma cell line, were transduced with expression plasmids encoding wild-type, kinase-inactivated or activated Fes. These cells were then engrafted into the mammary fat pads of nude mice and tumorigenesis was assessed by ultrasound (longitudinal tumor volume measurement), Doppler (tumor-associated vascular flow) and biophotonic imaging of GFP-expressing tumor cells (lung metastasis). There was no obvious difference in tumor growth and angiogenesis at the orthotopic site between each cell line and no apparent difference in lung metastasis. To test the potential stromal role of Fes in breast tumorigenesis, parental AC2M2 cells were injected into the mammary fat pads of wild type and Fes-null nude mice, and tumorigenesis and metastasis were assessed as described above. Wild type mice developed tumors significantly faster than Fes-null mice and there was earlier tumor neovascularization and more recruitment of macrophages. There was also a significantly higher degree of lung metastasis in wild type vs Fes-null mice. These observations are consistent with a tumor promoting role for Fes acting at the level of the vascular endothelium and macrophages. This might include promoting early tumor neovasularization through direct roles in vascular endothelial cells as well as indirect roles associated with macrophages. The increased metastatic potential also suggests roles in both these cell lineages. This provides genetic evidence that the Fes protein-tyrosine kinase represents a potential novel therapeutic target in breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3277.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».