209 EXPRESSION OF GENES RELATED TO OVULATORY CAPACITY (LHR AND AGTR2) IN GRANULOSA CELLS FROM SUPERSTIMULATED OR NOT SUPERSTIMULATED ANGUS COWS
Notice bibliographique
Résumé
Multiple ovulation and embryo transfer (MOET) has contributed to genetic improvement of Brazilian cattle and the P36 superstimulatory treatment protocol has been used successfully in MOET programs. Recently, increased mRNA levels of LH receptor (LHR) and angiotensin II receptor type 2 (AGTR2) have been reported in granulosa cells (GC) of superstimulated Nelore cows using P36 protocol and the efficacy of the P36 protocol has been investigated with the addition of a LH stimulus (eCG or LH), on the last day of treatment. We aimed to evaluate mRNA abundance of AGTR2 and LHR, related to ovulatory capacity, in GC of superstimulated Angus cows by different variations of the P36 protocol. Multiparous Angus cows (n = 37) were randomly assigned to four groups: control, P36, P36+eCG, and P36/FSH+LH. The control group (non-superstimulated cows, n = 7) received an intravaginal device containing progesterone (1.0 g; Primer®; Agener Animal Health, São Paulo, SP, Brazil) and 2.5 mg of oestradiol benzoate (EB; RIC-BE®; Agener Animal Health; IM) on a random day of the oestrous cycle (Day 0). After 8 days (Day 8) cows were treated with PGF2a (150 mg d-cloprostenol; Prolise®; Agener Animal Health; IM; 7:00 a.m.) and the intravaginal device was removed (7:00 p.m.). Cows from P36 group (n = 10) received the intravaginal device and 2.5 mg of EB at the beginning of the protocol (Day 0). Starting 5 days later (Day 5), 200 mg pFSH (Folltropin®; Bioniche Animal Health, Belleville, ON, Canada) was administered twice daily by IM injections over 4 days; on Day 7, PGF2a was administered and the intravaginal device was removed 36 h later (Day 8, 7:00 p.m.). Groups with the addition of a LH stimulus (eCG or LH administration) were submitted to the P36 protocol with replacement of the last two FSH doses by 200 IU eCG (P36+eCG; Novormon®, MSD Animal Health, São Paulo, SP, Brazil, IM; n = 10) or 2 mg pLH concomitant with the last two doses of FSH (P36/FSH+LH; Lutropin®, Bioniche Animal Health, IM; n = 10). Cows in all groups were slaughtered on Day 9, 12 h after progesterone-device removal and ovaries were transported on ice to the laboratory immediately after slaughter. Non-superstimulated dominant follicles and superstimulated follicles were dissected from the ovaries to obtain GC and total RNA was extracted. Amplification of housekeeping (peptidyl-prolyl isomerase A; PPIA) and target genes was performed by real-time RT–PCR according to the Sybr Green protocol. Relative gene expression was determined by Pfaffl's equation. The mRNA abundance was compared by Tukey's test and the significance level was P < 0.05. In GC, the mRNA abundance (mean ± s.e.m.) of LHR and AGTR2 was higher in the control group (1.02 ± 0.24 and 3.76 ± 1.07, respectively; P < 0.05) when compared with the superstimulated groups: P36 (0.33 ± 0.24 and 1.02 ± 0.21), P36+eCG (0.42 ± 0.10 and 1.40 ± 0.33) and P36/FSH+LH (0.38 ± 0.07 and 1.45 ± 0.49). In conclusion, the superstimulatory treatments decrease mRNA levels of LHR and AGTR2. We acknowledge CAPES for E. Lucacin's scholarship and FAPESP–São Paulo Research Foundation for grant number 2011/50593-2.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».