The Muscle-specific Calmodulin-dependent Protein Kinase Assembles with the Glycolytic Enzyme Complex at the Sarcoplasmic Reticulum and Modulates the Activity of Glyceraldehyde-3-phosphate Dehydrogenase in a Ca2+/Calmodulin-dependent Manner

The skeletal muscle specific Ca2+/calmodulin-dependent protein kinase (CaMKIIβM) is localized to the sarcoplasmic reticulum (SR) by an anchoring protein, αKAP, but its function remains to be defined. Protein interactions of CaMKIIβM indicated that it exists in complex with enzymes involved in glycolysis at the SR membrane. The kinase was found to complex with glycogen phosphorylase, glycogen debranching enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and creatine kinase in the SR membrane. CaMKIIβM was also found to assemble with aldolase A, GAPDH, enolase, lactate dehydrogenase, creatine kinase, pyruvate kinase, and phosphorylase b kinase from the cytosolic fraction. The interacting proteins were substrates of CaMKIIβM, and their was in and The CaMKIIβM and its in that the muscle CaMKIIβM to assemble the and enzymes at the SR and the of in to the of CaMKIIβM in to to and and at the SR in The skeletal muscle specific Ca2+/calmodulin-dependent protein kinase (CaMKIIβM) is localized to the sarcoplasmic reticulum (SR) by an anchoring protein, αKAP, but its function remains to be defined. Protein interactions of CaMKIIβM indicated that it exists in complex with enzymes involved in glycolysis at the SR membrane. The kinase was found to complex with glycogen phosphorylase, glycogen debranching enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and creatine kinase in the SR membrane. CaMKIIβM was also found to assemble with aldolase A, GAPDH, enolase, lactate dehydrogenase, creatine kinase, pyruvate kinase, and phosphorylase b kinase from the cytosolic fraction. The interacting proteins were substrates of CaMKIIβM, and their was in and The CaMKIIβM and its in that the muscle CaMKIIβM to assemble the and enzymes at the SR and the of in to the of CaMKIIβM in to to and and at the SR in by an of by kinase, protein GAPDH, glyceraldehyde-3-phosphate sarcoplasmic with lactate kinase, protein GAPDH, glyceraldehyde-3-phosphate sarcoplasmic with lactate is the The complex protein kinase is that is in and The kinase is to in and of of cytosolic and in skeletal an of is to the SR by protein, to kinase proteins by of its and SR the and the in skeletal muscle SR were to be substrates of to be the of of proteins by is that the and by and and at the and the of is to be phosphorylase creatine kinase, and that is to an at the is to the function the is to the SR and be in to the and muscle remains to be defined. also that the of the of the SR in at the SR be remains the of the SR to of the enzymes of the GAPDH, pyruvate kinase, and enzymes involved in glycogen phosphorylase b kinase and the debranching that CaMKIIβM proteins and the of in of the that and in the of SR function and that the CaMKIIβM be the to the SR and the of and in to in skeletal of and from skeletal muscle was with the of the of The was by and and with from was at in and by in at The were to and were and by in were by and were the by an and and with of and skeletal muscle the were with the protein in in proteins were with The proteins were in of and SR of SR was muscle from was in of and and in and at The muscle were in and in The muscle was in with The was at and the was and at The was at cytosolic was and SR was in and and with of SR proteins were and at Protein of was to kinase was with kinase protein was to in at the of of the were with and and was by was at by the of at in an and The was by an in the of with CaMKIIβM, the was by of was by the in at in was in and to and in were in at and the was by of kinase was in an by the from the of by and The pyruvate and of in at The was by of was by pyruvate in the of with in and at The of was by in at phosphorylase was by and The of and of of the of CaMKIIβM the of were in the of in the were by and CaMKIIβM to proteins were with SR cytosolic proteins in of with at The in the were to that by were in were with and by with and were at in in of of SR cytosolic protein, of and the indicated of were by in and by in and protein were were in in with and with with was an of of proteins of CaMKIIβM was by proteins in the were to at in and the the was by to the and proteins were and of protein was also by with protein at by the of of the were with and was by the protein kinase in the by were and to to in the of The were and was and with at an of by at the of CaMKIIβM in of CaMKIIβM was by of SR and cytosolic from skeletal muscle with CaMKIIβM of in SR but the cytosolic with that The kinase was protein, and the of CaMKIIβM was with of and also by of CaMKIIβM was in an kinase and indicated an of in in the of and to of the the of the SR CaMKIIβM, of the interacting proteins was with cytosolic and SR from skeletal of the interacting proteins with to that of and from the and of and from the SR was of to and in an with the protein and of and also and The kinase and its in in and and in with The protein in the in of the in and of was in of the was and the of CaMKIIβM with enzymes of and glycogen The interacting proteins from cytosolic and were to be and enzymes of the aldolase A, GAPDH, enolase, and pyruvate kinase, from the SR and an with glycogen phosphorylase glycogen debranching enzyme, creatine kinase, and The that and creatine kinase were interacting proteins in cytosolic and SR interacting of in the with the SR proteins and of in with cytosolic proteins were to be the of the kinase of enzymes is to and with the of of and from cytosolic were also the proteins were were by phosphorylase b kinase and of the complex in skeletal debranching kinase kinase kinase kinase kinase in The of the indicated specific with the of the glycolysis were by The proteins in the were in in the and of and and the is in of the interacting proteins were in in cytosolic and SR of skeletal muscle proteins to the of and from the were pyruvate kinase, enolase, creatine kinase, and GAPDH, and the and from the SR to glycogen phosphorylase and GAPDH, were the protein at and b and found in with and the interacting proteins was by of CaMKIIβM protein and its is and with cytosolic and SR and SR proteins from skeletal muscle were with protein proteins were in in in and with in of cytosolic in of cytosolic in of SR in of SR in and the of CaMKIIβM and its in the of proteins substrates of the of were with cytosolic and SR proteins and to in the of and proteins were in of CaMKIIβM and its in of proteins from cytosolic and of proteins from SR of the proteins was by the protein with CaMKIIβM in the and of and in indicated that pyruvate kinase, and were substrates of with that that GAPDH, and pyruvate kinase with the SR and by the the of the of the the of the enzymes was in the and of and in kinase The in that was of the of pyruvate kinase, creatine kinase, and glycogen phosphorylase the of was by CaMKIIβM in the of of of the of of was at pyruvate lactate GAPDH, glyceraldehyde-3-phosphate glycogen creatine of was at of was at of was at of was at of was at The of was at pyruvate lactate GAPDH, glyceraldehyde-3-phosphate glycogen creatine in the SR is to and the CaMKIIβM is to the to in the in with the cytosolic and SR proteins were by with and the of in cytosolic and SR with the in skeletal muscle were and from cytosolic and SR that the CaMKIIβM protein that is by the from cytosolic and SR The of from cytosolic SR and to that with the the and the kinase protein and The was and with protein and interactions with muscle and SR were and of protein were by by with of and with cytosolic and SR proteins were in were and to with in in of cytosolic of cytosolic of SR of SR The the and with of the of the CaMKIIβM and the to with GAPDH, with the SR fraction. with that CaMKIIβM and and CaMKIIβM with the and The was and with protein in the The from was by and that of was in complex with the CaMKIIβM with the the of CaMKIIβM and in skeletal muscle SR with of SR was the of that kinase CaMKIIβM from SR by by The complex also the that was by CaMKIIβM with GAPDH, with and and in with The was to and with with was at by with and of and of interactions of CaMKIIβM and the from with of SR proteins were with and and was with in in of SR of SR of SR of SR The with and the the and with and by the and muscle specific of with were by by and with The was and with of with was with and by with The of in was by with of at CaMKIIβM GAPDH, the of and of by the of the CaMKIIβM found that at of at in an in by CaMKIIβM and of and to of of of was by in of and of by of by CaMKIIβM was with the of was at of and was at of of indicated were in the of and was protein of CaMKIIβM, and was with at of was with at in to was The of at to the the of the SR in skeletal muscle to CaMKIIβM was to be to the SR in skeletal muscle by protein, but the of kinase in SR function remains found that CaMKIIβM with the enzymes of glycolysis and glycogen The glycogen phosphorylase, glycogen debranching enzyme, GAPDH, and creatine kinase interacting proteins from the SR and aldolase A, GAPDH, enolase, creatine kinase, pyruvate kinase, and phosphorylase b kinase from the cytosolic fraction. indicated that glycogen phosphorylase at the SR in with glycogen and its to that CaMKIIβM complex with phosphorylase b kinase at the and the to be by also the glycogen debranching at the in glycogen the that CaMKIIβM with to and its in and was found to be in muscle and the SR and also that with enzymes to the of the SR in skeletal muscle with kinase in the of from and at the of the SR that were of the the of the is that found to with but to with the of the that to the SR in muscle and in to in to in to the in and of enzymes in to the and in complex and at the SR were to in is and in the the glycogen and enzymes in complex with CaMKIIβM at the SR and in complex SR function The of with the CaMKIIβM at the SR in the of to the function of membrane. from the SR to the of phosphorylase b kinase glycogen to the phosphorylase b and debranching The also CaMKIIβM, and of in and at the SR membrane. the and the The also to and be of in muscle in and the of CaMKIIβM the of SR function in to of CaMKIIβM with the complex at the CaMKIIβM is to SR by CaMKIIβM GAPDH, with aldolase and kinase from phosphorylase b kinase, glycogen to by glycogen phosphorylase and glycogen debranching enzymes also CaMKIIβM, is to by and is by of to and and GAPDH, glyceraldehyde-3-phosphate glycogen phosphorylase phosphorylase b creatine sarcoplasmic by an of by kinase, protein GAPDH, glyceraldehyde-3-phosphate sarcoplasmic with lactate kinase, protein GAPDH, glyceraldehyde-3-phosphate sarcoplasmic with lactate is the The complex protein kinase is that is in and The kinase is to in and of The of cytosolic and in skeletal an of is to the SR by protein, to kinase proteins by of its and SR the and the in skeletal muscle SR were to be substrates of to be the of of proteins by is that the and by and and at the and the of is to be phosphorylase creatine kinase, and that is to an at the is to the function the is to the SR and be in to the and muscle remains to be defined. also that the of the of the SR in at the SR be remains the of the SR to of the enzymes of the GAPDH, pyruvate kinase, and enzymes involved in glycogen phosphorylase b kinase and the debranching that CaMKIIβM proteins and the of in of the that and in the of SR function and that the CaMKIIβM be the to the SR and the of and in to in skeletal of and from skeletal muscle was with the of the of The was by and and with from was at in and by in at The were to and were and by in were by and were the by an and and with of and skeletal muscle the were with the protein in in proteins were with The proteins were in of and SR of SR was muscle from was in of and and in and at The muscle were in and in The muscle was in with The was at and the was and at The was at cytosolic was and SR was in and and with of SR proteins were and at Protein of was to kinase was with kinase protein was to in at the of of the were with and and was by was at by the of at in an and The was by an in the of with CaMKIIβM, the was by of was by the in at in was in and to and in were in at and the was by of kinase was in an by the from the of by and The pyruvate and of in at The was by of was by pyruvate in the of with in and at The of was by in at phosphorylase was by and The of and of of the of CaMKIIβM the of were in the of in the were by and CaMKIIβM to proteins were with SR cytosolic proteins in of with at The in the were to that by were in were with and by with and were at in in of of SR cytosolic protein, of and the indicated of were by in and by in and protein were were in in with and with with was an of of proteins of CaMKIIβM was by proteins in the were to at in and the the was by to the and proteins were and of protein was also by with protein at by the of of the were with and was by the protein kinase in the by were and to to in the of The were and was and with at an of by at the of and from skeletal muscle was with the of the of The was by and and with from was at in and by in at The were to and were and by in were by and were the by an and and with of and skeletal muscle the were with the protein in in proteins were with The proteins were in of and SR of SR was muscle from was in of and and in and at The muscle were in and in The muscle was in with The was at and the was and at The was at cytosolic was and SR was in and and with of SR proteins were and at Protein of was to kinase was with kinase protein was to in at the of of the were with and and was by was at by the of at in an and The was by an in the of with CaMKIIβM, the was by of was by the in at in was in and to and in were in at and the was by of kinase was in an by the from the of by and The pyruvate and of in at The was by of was by pyruvate in the of with in and at The of was by in at phosphorylase was by and The of and of of the of CaMKIIβM the of were in the of in the were by and CaMKIIβM to proteins were with SR cytosolic proteins in of with at The in the were to that by were in were with and by with and were at in in of of SR cytosolic protein, of and the indicated of were by in and by in and protein were were in in with and with with was an of of proteins of CaMKIIβM was by proteins in the were to at in and the the was by to the and proteins were and of protein was also by with protein at by the of of the were with and was by the protein kinase in the Protein by were and to to in the of The were and was and with at an of by at the of CaMKIIβM in of CaMKIIβM was by of SR and cytosolic from skeletal muscle with CaMKIIβM of in SR but the cytosolic with that The kinase was protein, and the of CaMKIIβM was with of and also by of CaMKIIβM was in an kinase and indicated an of in in the of and to of the the of the SR CaMKIIβM, of the interacting proteins was with cytosolic and SR from skeletal of the interacting proteins with to that of and from the and of and from the SR was of to and in an with the protein and of and also and The kinase and its in in and and in with The protein in the in of the in and of was in of the was and the of CaMKIIβM with enzymes of and glycogen The interacting proteins from cytosolic and were to be and enzymes of the aldolase A, GAPDH, enolase, and pyruvate kinase, from the SR and an with glycogen phosphorylase glycogen debranching enzyme, creatine kinase, and The that and creatine kinase were interacting proteins in cytosolic and SR interacting of in the with the SR proteins and of in with cytosolic proteins were to be the of the kinase of enzymes is to and with the of of and from cytosolic were also the proteins were were by phosphorylase b kinase and of the complex in skeletal debranching kinase kinase kinase kinase kinase in The of the indicated specific with the of the glycolysis were by The proteins in the were in in the and of and and the is in of the interacting proteins were in in cytosolic and SR of skeletal muscle proteins to the of and from the were pyruvate kinase, enolase, creatine kinase, and GAPDH, and the and from the SR to glycogen phosphorylase and GAPDH, were the protein at and b and found in with and the interacting proteins was by of CaMKIIβM protein and its is and proteins substrates of the of were with cytosolic and SR proteins and to in the of and proteins were in of CaMKIIβM and its in of proteins from cytosolic and of proteins from SR of the proteins was by the protein with CaMKIIβM in the and of and in indicated that pyruvate kinase, and were substrates of with that that GAPDH, and pyruvate kinase with the SR and by the the of the of the the of the enzymes was in the and of and in kinase The in that was of the of pyruvate kinase, creatine kinase, and glycogen phosphorylase the of was by CaMKIIβM in the of of of the of of was at pyruvate lactate GAPDH, glyceraldehyde-3-phosphate glycogen creatine of was at of was at of was at of was at of was at The of was at pyruvate lactate GAPDH, glyceraldehyde-3-phosphate glycogen creatine in the SR is to and the CaMKIIβM is to the to in the in with the cytosolic and SR proteins were by with and the of in cytosolic and SR with the in skeletal muscle were and from cytosolic and SR that the CaMKIIβM protein that is by the from cytosolic and SR The of from cytosolic SR and to that with the the and the kinase protein and The was and with protein and interactions with muscle and SR were and of protein were by by with of and with cytosolic and SR proteins were in were and to with in in of cytosolic of cytosolic of SR of SR The the and with of the of the CaMKIIβM and the to with GAPDH, with the SR fraction. with that CaMKIIβM and and CaMKIIβM with the and The was and with protein in the The from was by and that of was in complex with the CaMKIIβM with the the of CaMKIIβM and in skeletal muscle SR with of SR was the of that kinase CaMKIIβM from SR by by The complex also the that was by CaMKIIβM with GAPDH, with and and in with The was to and with with was at by with and of and of interactions of CaMKIIβM and the from with of SR proteins were with and and was with in in of SR of SR of SR of SR The with and the the and with and by the and muscle specific of with were by by and with The was and with of with was with and by with The of in was by with of at CaMKIIβM GAPDH, the of and of by the of the CaMKIIβM found that at of at in an in by CaMKIIβM and of and to of of of was by in of and of by of by CaMKIIβM was with the of was at of and was at of of indicated were in the of and was protein of CaMKIIβM, and was with at of was with at in to was The of at of CaMKIIβM in of CaMKIIβM was by of SR and cytosolic from skeletal muscle with CaMKIIβM of in SR but the cytosolic with that The kinase was protein, and the of CaMKIIβM was with of and also by The of CaMKIIβM was in an kinase and indicated an of in in the of and to of the the of the SR CaMKIIβM, of the interacting proteins was with cytosolic and SR from skeletal of the interacting proteins with to that of and from the and of and from the SR was of to and in an with the protein and of and also and The kinase and its in in and and in with The protein in the in of the in and of was in The of the was and the of CaMKIIβM with enzymes of and glycogen The interacting proteins from cytosolic and were to be and enzymes of the aldolase A, GAPDH, enolase, and pyruvate kinase, from the SR and an with glycogen phosphorylase glycogen debranching enzyme, creatine kinase, and The that and creatine kinase were interacting proteins in cytosolic and SR interacting of in the with the SR proteins and of in with cytosolic proteins were to be the of the kinase of enzymes is to and with the of of and from cytosolic were also the proteins were were by phosphorylase b kinase and The of the indicated specific with the of the glycolysis were by The proteins in the were in in the and of and and the is in of the interacting proteins were in in cytosolic and SR of skeletal muscle proteins to the of and from the were pyruvate kinase, enolase, creatine kinase, and GAPDH, and the and from the SR to glycogen phosphorylase and GAPDH, were the protein at and b and found in with and the interacting proteins was by of CaMKIIβM protein and its is and The of the proteins was by the protein with CaMKIIβM in the and of and in indicated that pyruvate kinase, and were substrates of with that that GAPDH, and pyruvate kinase with the SR and by the CaMKIIβM the of the of the the of the enzymes was in the and of and in kinase The in that was of the of pyruvate kinase, creatine kinase, and glycogen phosphorylase the of was by CaMKIIβM in the of the SR is to and the CaMKIIβM is to the to in the in with the cytosolic and SR proteins were by with and the of in cytosolic and SR with the in skeletal muscle were and from cytosolic and SR that the CaMKIIβM protein that is by the from cytosolic and SR The of from cytosolic SR and to that with the the and the kinase protein and The was and with protein the of the CaMKIIβM and the to with GAPDH, with the SR fraction. with that CaMKIIβM and and CaMKIIβM with the and The was and with protein in the The from was by and that of was in complex with the CaMKIIβM with the the of CaMKIIβM and in skeletal muscle SR with of SR was the of that kinase CaMKIIβM from SR by by The complex also the that was by CaMKIIβM with GAPDH, with and and in with The was to and with with was at by with and CaMKIIβM GAPDH, the of and of by the of the CaMKIIβM found that at of at in an in by CaMKIIβM and of and to of of of was by in of to the the of the SR in skeletal muscle to CaMKIIβM was to be to the SR in skeletal muscle by protein, but the of kinase in SR function remains found that CaMKIIβM with the enzymes of glycolysis and glycogen The glycogen phosphorylase, glycogen debranching enzyme, GAPDH, and creatine kinase interacting proteins from the SR and aldolase A, GAPDH, enolase, creatine kinase, pyruvate kinase, and phosphorylase b kinase from the cytosolic fraction. indicated that glycogen phosphorylase at the SR in with glycogen and its to that CaMKIIβM complex with phosphorylase b kinase at the and the to be by also the glycogen debranching at the in glycogen the that CaMKIIβM with to and its in and was found to be in muscle and the SR and also that with enzymes to the of the SR in skeletal muscle with kinase in the of from and at the of the SR that were of the the of the is that found to with but to with the of the that to the SR in muscle and in to in to in to the in and of enzymes in to the and in complex and at the SR were to in is and in the the glycogen and enzymes in complex with CaMKIIβM at the SR and in complex SR function The of with the CaMKIIβM at the SR in the of to the function of membrane. from the SR to the of phosphorylase b kinase glycogen to the phosphorylase b and debranching The also CaMKIIβM, and of in and at the SR membrane. the and the The also to and be of in muscle in and the of CaMKIIβM the of SR function in to to the the of the SR in skeletal muscle to CaMKIIβM was to be to the SR in skeletal muscle by protein, but the of kinase in SR function remains found that CaMKIIβM with the enzymes of glycolysis and glycogen The glycogen phosphorylase, glycogen debranching enzyme, GAPDH, and creatine kinase interacting proteins from the SR and aldolase A, GAPDH, enolase, creatine kinase, pyruvate kinase, and phosphorylase b kinase from the cytosolic fraction. indicated that glycogen phosphorylase at the SR in with glycogen and its to that CaMKIIβM complex with phosphorylase b kinase at the and the to be by also the glycogen debranching at the in glycogen the that CaMKIIβM with to and its in and was found to be in muscle and the SR and also that with enzymes to the of the SR in skeletal muscle with kinase in the of from and at the of the SR that were of the the of the is that found to with but to with the of the that to the SR in muscle and in to in to in to the in and of enzymes in to the and in complex and at the SR were to in is and in the the glycogen and enzymes in complex with CaMKIIβM at the SR and in complex SR function The of with the CaMKIIβM at the SR in the of to the function of membrane. from the SR to the of phosphorylase b kinase glycogen to the phosphorylase b and debranching The also CaMKIIβM, and of in and at the SR membrane. the and the The also to and be of in muscle in and the of CaMKIIβM the of SR function in to

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.