368 OOCYTE ASPIRATION FROM TRANSITIONAL AND CYCLING MARES TREATED WITH eFSH
Notice bibliographique
Résumé
A major obstacle in studying equine oocyte maturation is a source of oocytes. The aim of this study was to determine if oocyte recovery following transvaginal ultrasound-guided aspirations could be improved by using eFSH. Fourteen mares ranging from 3 to 16 years of age were used as oocyte donors during the transitional (March to April) and cycling (May to June) seasons. Each follicle was aspirated with a 12-gauge double-lumen needle using a pressure of 150 mmHg and then flushed eight times using PBS supplemented with 10 U/mL heparin and 0.05% BSA. Large follicles (>20 mm) were aspirated first, followed by small follicles (10–20 mm). The first aspiration in each season was used to deplete the follicular pool of all follicles >10 mm; these data were not considered. Following this initial aspiration, mares were subjected to four rounds of aspirations every 10 to 11 days. In each round during the transitional season, half the mares received 12.5 mg eFSH (Bioniche Animal Health, Belleville, Ontario, Canada) once daily for four days prior to aspiration. For each round during the cycling season, half the mares received 12.5 mg eFSH twice daily for three days prior to aspiration. In each replicate, mares were alternated from treated to control groups during both seasons. Oocyte recovery and superstimulatory responses were analyzed using the GLM procedure of SAS (SAS Institute, Inc., Cary, NC, USA). During both the transitional and the cycling season, treatment with eFSH did not have a significant effect (P > 0.1) on the number of small or large follicles present at the time of aspiration (Table 1). For both seasons and size classes, the number of follicles varied by mare (P < 0.02). Oocyte recovery from small follicles during the transitional season improved during the later two rounds of aspiration (0.58 vs. 1.49; P < 0.01) and was also affected by mare (P = 0.06). During the cycling season, recovery of oocytes from both small and large follicles varied by mare (P < 0.05). Treatment with eFSH increased the number of oocytes recovered from large follicles in cycling mares (P < 0.05). This study indicates that responses to short-term eFSH treatment for the purpose of oocyte recovery vary greatly by mare. While treatment with eFSH did not increase the number of follicles available for aspiration (P > 0.1), it did increase the number of oocytes recovered from large follicles during the cycling season. Table 1. Oocyte recovery and superstimulatory response of mares treated with eFSH
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».