Integrin-induced Tyrosine Phosphorylation of Protein-tyrosine Phosphatase-α Is Required for Cytoskeletal Reorganization and Cell Migration

Protein-tyrosine phosphatase-α (PTPα) activates Src family kinases (SFKs) to promote the integrin-stimulated early autophosphorylation of focal adhesion kinase (FAK). We report here that integrin stimulation induces tyrosine phosphorylation of PTPα. PTPα was dephosphorylated upon fibroblast detachment from the substratum and rephosphorylated when cells were plated on the integrin ligand fibronectin. α PTP phosphorylation occurred at Tyr789 and required SFKs (Src and required PTPα that PTPα activates SFKs and that the tyrosine kinase PTPα. of PTPα and and PTPα and focal adhesion of PTPα integrin early of SFKs and PTPα phosphorylation and PTPα phosphorylation at Tyr789 Protein-tyrosine phosphatase-α (PTPα) activates Src family kinases (SFKs) to promote the integrin-stimulated early autophosphorylation of focal adhesion kinase (FAK). We report here that integrin stimulation induces tyrosine phosphorylation of PTPα. PTPα was dephosphorylated upon fibroblast detachment from the substratum and rephosphorylated when cells were plated on the integrin ligand fibronectin. α PTP phosphorylation occurred at Tyr789 and required SFKs (Src and required PTPα that PTPα activates SFKs and that the tyrosine kinase PTPα. of PTPα and and PTPα and focal adhesion of PTPα integrin early of SFKs and PTPα phosphorylation and PTPα phosphorylation at Tyr789 of the required the of of tyrosine of the focal adhesion kinase focal adhesion Src family Src fibronectin. focal adhesion Src family Src fibronectin. and the of Src family kinases (SFKs) phosphorylation of and the of the tyrosine kinase phosphorylation and of phosphorylation of at that to and phosphorylation of and to promote and and that focal adhesion and and and to the and phosphorylation of at of at autophosphorylation phosphorylation SFKs that at and to and promote autophosphorylation of SFKs the autophosphorylation to integrin the SFKs and cells the of the to the and of the to the and kinase the tyrosine the that to and the of autophosphorylation of and kinase of the and SFKs to Src early autophosphorylation Src and the of Src to promote Src and Src the Src Src to Src autophosphorylation and upon integrin SFKs integrin the that of kinases of the phosphatase-α of SFKs phosphorylation that PTPα of of SFKs phosphorylation and that PTPα and activates and Src of the of Src PTPα and of integrin integrin PTPα to to We report here that integrin stimulation the tyrosine phosphorylation of PTPα at of the of integrin that required of SFKs and focal adhesion and and PTPα integrin early autophosphorylation and that of PTPα tyrosine phosphorylation and focal adhesion and PTPα tyrosine phosphorylation that the and and were from the and were at the and fibroblast were from cells were and to stimulation cells were cells were of was and of cells on and was of the the and the PTPα was and was from the from and cells were and to and were from to and and Src and were from and were from was from was from and were from was were the to the from were to of of was the on and were and were to and at the were and at were and and was and the cells were the cells were and at cells were plated and at were and of were to and cells were of and to of at the cells were and and were and and and on were and at the cells were was and were and were and was PTPα PTPα and was the was and the was of was and were the and of the were and to the the of PTPα were and were to the were and cells were from the cells and were were of that the at the was to the and the cells were to of was fibroblast and PTPα phosphorylation was adhesion and integrin were and plated on the integrin ligand of cells from the substratum the of PTPα of that of PTPα and and and on phosphorylation of PTPα to of the cells on to of the on and and the cells on of PTPα and the the tyrosine phosphorylation of PTPα that of the that was the PTPα upon the of PTPα to the of PTPα tyrosine phosphorylation We the upon integrin PTPα and Src and the of PTPα required were PTPα the of PTPα Tyr789 PTPα was and PTPα were of phosphorylation of PTPα that PTPα required to to PTPα of PTPα and cells tyrosine phosphorylation of cells and cells plated on of and phosphorylation of cells to the plated on that the phosphorylation of of PTPα the of Src to the of of integrin-stimulated phosphorylation at PTPα Src required phosphorylation of PTPα at that were were PTPα were on were on and on PTPα were on and on PTPα were from the and PTPα were were were to PTPα and PTPα and were the were PTPα the cells were and plated on were to PTPα and PTPα that Tyr789 was the PTPα upon integrin the of PTPα. and were from cells were the PTPα was PTPα were and plated on to PTPα tyrosine phosphorylation and cells PTPα was of cells cells that the PTPα at Tyr789 and that upon integrin PTPα that PTPα phosphorylation SFKs was PTPα was dephosphorylated when Src were and was rephosphorylated when the cells were plated on that Src PTPα the SFKs and PTPα phosphorylation was from the substratum and on PTPα phosphorylation cells Src and PTPα was dephosphorylated when the cells were and rephosphorylated when the cells were plated on on PTPα tyrosine phosphorylation were when from cells of were of of and that integrin-stimulated phosphorylation of PTPα at required PTPα tyrosine Src and and were on the of and and to on PTPα from were PTPα from cells plated on were to PTPα and PTPα of and and was of and to PTPα induces and the of the were PTPα and was and of PTPα detachment was and tyrosine phosphorylation of PTPα was the of and phosphorylation of at and and that required PTPα integrin-stimulated phosphorylation the required PTPα of and and PTPα phosphorylation when the were and on of PTPα phosphorylation the cells was to that the cells and that the tyrosine kinase PTPα phosphorylation and that PTPα phosphorylation of tyrosine phosphorylation of PTPα on were to cells were and of the cells were on the of PTPα was from the and and PTPα was from the and and PTPα tyrosine phosphorylation and were on and on PTPα was from the and and PTPα Tyr789 at early integrin required integrin-stimulated phosphorylation of at PTPα activates and PTPα was to the of PTPα to PTPα Tyr789 phosphorylation required integrin-stimulated and the tyrosine phosphorylation of cells of PTPα PTPα and tyrosine phosphorylation were of of PTPα cells PTPα was and tyrosine phosphorylation was cells cells and and on phosphorylation were to phosphorylation Src phosphorylation was Src the Src dephosphorylated at Src of phosphorylation and the of Src were Src was cells phosphorylation the of Src phosphorylation of Src at cells cells of the of phosphorylation of cells of PTPα of Src at phosphorylation of Src at and phosphorylation of at to the the of PTPα on Src phosphorylation and was to phosphorylation of cells Src and tyrosine phosphorylation to and to of α PTP PTPα Tyr789 required PTPα to phosphorylation of integrin of PTPα induces phosphorylation (Src and Src that phosphorylation of PTPα required integrin-stimulated and and and PTPα were and PTPα tyrosine the cells were and plated on to of Src and and the to tyrosine phosphorylation the were the that and were the to and tyrosine phosphorylation of and the of were and the phosphorylation of Src and and and of the cells was of and were to required integrin-stimulated phosphorylation and of PTPα cells integrin-stimulated phosphorylation of at to that cells phosphorylation at that cells of and the kinase were upon of PTPα and that phosphorylation of PTPα at Tyr789 required early required to integrin-stimulated and tyrosine PTPα the of Tyr789 cells on of and focal of PTPα the cells We the of PTPα and phosphorylation at required of integrin-stimulated cells were plated on and and the cells were and to and on was that cells PTPα on the substratum and of and the to cells and that was the the cells to on and the cells that of and was cells cells PTPα. on cells cells PTPα were and and focal cells and cells was at were cells PTPα cells and and of and focal adhesion of the cells PTPα to and the cells and and PTPα that the of PTPα and phosphorylation at Tyr789 integrin-stimulated and focal adhesion PTPα Tyr789 and focal adhesion cells PTPα and and PTPα were and plated on and were and and and integrin-stimulated that required cells to of cells that of PTPα of cells PTPα of cells the of to and focal adhesion PTPα PTPα was to of cells the cells of cells PTPα and and PTPα were the of of cells was and were to was the of cells and cells cells cells and cells PTPα were the of cells and cells PTPα cells and cells that PTPα tyrosine phosphorylation to integrin PTPα dephosphorylated when cells and rephosphorylated when cells plated on fibronectin. phosphorylation at Tyr789 the of PTPα and Src PTPα and activates SFKs and of SFKs early integrin PTPα Src and and PTPα tyrosine and the autophosphorylation required that required the phosphorylation of PTPα at Tyr789 that integrin required PTPα phosphorylation that PTPα phosphorylation of the and required integrin-stimulated PTPα tyrosine phosphorylation phosphorylation and and PTPα tyrosine phosphorylation to that PTPα Tyr789 phosphorylation required was PTPα Src and of and and and to that phosphorylation of PTPα at Tyr789 to and cells was to to Src at of Src that of the PTPα to the that were of PTPα of the was to to to of Src at and of Src of Src was the Src to of the of Src and the of PTPα and Src to of Src at PTPα of PTPα was to α and that Src and Src that PTPα Tyr789 phosphorylation required integrin-stimulated to PTPα We that integrin that the PTPα of PTPα and Src the the of PTPα to SFKs to that the PTPα to ligand the integrin the Src the the Src of Src to the of the Src that of Src to Src integrin that and PTPα to promote and PTPα tyrosine of PTPα integrin to integrin SFKs upon integrin to SFKs integrin of the of the PTPα integrin and SFKs SFKs at and to and promote on and and PTPα at the and focal adhesion PTPα Tyr789 phosphorylation required phosphorylation and tyrosine phosphorylation of the required integrin on and the of and of focal the of PTPα of the the to that tyrosine phosphorylation of PTPα required of PTPα that at integrin to and focal adhesion that PTPα of SFKs PTPα phosphorylation and the stimulation activates and to and the of and activates to promote and the of the of focal adhesion that and and focal adhesion cells that PTPα phosphorylation the of of of of PTPα the that PTPα the Tyr789 PTPα required PTPα to to focal of the focal adhesion to that from to the integrin the of PTPα and Src the that of of PTPα integrin PTPα early of the SFKs Src and phosphorylation and autophosphorylation at tyrosine phosphorylation of and PTPα early upon the of PTPα and to the of Src and We that phosphorylation of PTPα at Tyr789 Src required integrin the phosphorylation of PTPα required the of of to focal adhesion and of the required the of of tyrosine of the focal adhesion kinase focal adhesion Src family Src fibronectin. focal adhesion Src family Src fibronectin. and the of Src family kinases (SFKs) phosphorylation of and the of the tyrosine kinase phosphorylation and of phosphorylation of at that to and phosphorylation of and to promote and and that focal adhesion and and and to the and phosphorylation of at of at autophosphorylation phosphorylation SFKs that at and to and promote autophosphorylation of SFKs the autophosphorylation to integrin the SFKs and cells the of the to the and of the to the and kinase the tyrosine the that to and the of autophosphorylation of and kinase of the and SFKs to Src early autophosphorylation Src and the of Src to promote Src and Src the Src Src to Src autophosphorylation and upon integrin SFKs integrin the that of kinases of the phosphatase-α of SFKs phosphorylation that PTPα of of SFKs phosphorylation and that PTPα and activates and Src of the of Src PTPα and of integrin integrin PTPα to to We report here that integrin stimulation the tyrosine phosphorylation of PTPα at of the of integrin that required of SFKs and focal adhesion and and PTPα integrin early autophosphorylation and that of PTPα tyrosine phosphorylation and focal adhesion and PTPα tyrosine phosphorylation that the and and were from the and were at the and fibroblast were from cells were and to stimulation cells were cells were of was and of cells on and was of the the and the PTPα was and was from the from and cells were and to and were from to and and Src and were from and were from was from was from and were from was were the to the from were to of of was the on and were and were to and at the were and at were and and was and the cells were the cells were and at cells were plated and at were and of were to and cells were of and to of at the cells were and and were and and and on were and at the cells were was and were and were and was PTPα PTPα and was the was and the was of was and were the and of the were and to the the of PTPα were and were to the were and cells were from the cells and were were of that the at the was to the and the cells were to and and were from the and were at the and fibroblast were from cells were and to stimulation cells were cells were of was and of cells on and was of the the and the PTPα was and was from the from and cells were and to and were from to and and Src and were from and were from was from was from and were from was were the to the from were to of of was the on and were and were to and at the were and at were and and was and the cells were the cells were and at cells were plated and at were and of were to and cells were of and to of at the cells were and and were and and and on were and at the cells were was and were and were and PTPα was PTPα PTPα and was the was and the was of was and were the and of the were and to the the of PTPα were and were to the were and cells were from the cells and were were of that the at the was to the and the cells were to of was fibroblast and PTPα phosphorylation was adhesion and integrin were and plated on the integrin ligand of cells from the substratum the of PTPα of that of PTPα and and and on phosphorylation of PTPα to of the cells on to of the on and and the cells on of PTPα and the the tyrosine phosphorylation of PTPα that of the that was the PTPα upon the of PTPα to the of PTPα tyrosine phosphorylation We the upon integrin PTPα and Src and the of PTPα required were PTPα the of PTPα Tyr789 PTPα was and PTPα were of phosphorylation of PTPα that PTPα required to to PTPα of PTPα and cells tyrosine phosphorylation of cells and cells plated on of and phosphorylation of cells to the plated on that the phosphorylation of of PTPα the of Src to the of of integrin-stimulated phosphorylation at that Tyr789 was the PTPα upon integrin the of PTPα. and were from cells were the PTPα was PTPα were and plated on to PTPα tyrosine phosphorylation and cells PTPα was of cells cells that the PTPα at Tyr789 and that upon integrin PTPα that PTPα phosphorylation SFKs was PTPα was dephosphorylated when Src were and was rephosphorylated when the cells were plated on that Src PTPα the SFKs and PTPα phosphorylation was from the substratum and on PTPα phosphorylation cells Src and PTPα was dephosphorylated when the cells were and rephosphorylated when the cells were plated on on PTPα tyrosine phosphorylation were when from cells of were of of and that integrin-stimulated phosphorylation of PTPα at required PTPα tyrosine Src and and were on the of and and to on PTPα from were PTPα from cells plated on were to PTPα and PTPα of and and was of and to PTPα induces and the of the were PTPα and was and of PTPα detachment was and tyrosine phosphorylation of PTPα was the of and phosphorylation of at and and that required PTPα integrin-stimulated phosphorylation the required PTPα of and and PTPα phosphorylation when the were and on of PTPα phosphorylation the cells was to that the cells and that the tyrosine kinase PTPα phosphorylation and that PTPα phosphorylation of tyrosine phosphorylation of PTPα on were to cells were and of the cells were on the of PTPα was from the and and PTPα was from the and and PTPα tyrosine phosphorylation and were on and on PTPα was from the and and PTPα Tyr789 at early integrin required integrin-stimulated phosphorylation of at PTPα activates and PTPα was to the of PTPα to PTPα Tyr789 phosphorylation required integrin-stimulated and the tyrosine phosphorylation of cells of PTPα PTPα and tyrosine phosphorylation were of of PTPα cells PTPα was and tyrosine phosphorylation was cells cells and and on phosphorylation were to phosphorylation Src phosphorylation was Src the Src dephosphorylated at Src of phosphorylation and the of Src were Src was cells phosphorylation the of Src phosphorylation of Src at cells cells of the of phosphorylation of cells of PTPα of Src at phosphorylation of Src at and phosphorylation of at to the the of PTPα on Src phosphorylation and was to phosphorylation of cells Src and tyrosine phosphorylation to and to of α PTP PTPα Tyr789 required PTPα to phosphorylation of integrin of PTPα induces phosphorylation (Src and Src that phosphorylation of PTPα required integrin-stimulated and and and PTPα were and PTPα tyrosine the cells were and plated on to of Src and and the to tyrosine phosphorylation the were the that and were the to and tyrosine phosphorylation of and the of were and the phosphorylation of Src and and and of the cells was of and were to required integrin-stimulated phosphorylation and of PTPα cells integrin-stimulated phosphorylation of at to that cells phosphorylation at that cells of and the kinase were upon of PTPα and that phosphorylation of PTPα at Tyr789 required early required to integrin-stimulated and tyrosine PTPα the of Tyr789 cells on of and focal of PTPα the cells We the of PTPα and phosphorylation at required of integrin-stimulated cells were plated on and and the cells were and to and on was that cells PTPα on the substratum and of and the to cells and that was the the cells to on and the cells that of and was cells cells PTPα. on cells cells PTPα were and and focal cells and cells was at were cells PTPα cells and and of and focal adhesion of the cells PTPα to and the cells and and PTPα that the of PTPα and phosphorylation at Tyr789 integrin-stimulated and focal adhesion PTPα Tyr789 and focal adhesion cells PTPα and and PTPα were and plated on and were and and and integrin-stimulated that required cells to of cells that of PTPα of cells PTPα of cells the of to and focal adhesion PTPα PTPα was to of cells the cells of cells PTPα and and PTPα were the of of cells was and were to was the of cells and cells cells cells and cells PTPα were the of cells and cells PTPα cells and cells of was fibroblast and PTPα phosphorylation was adhesion and integrin were and plated on the integrin ligand of cells from the substratum the of PTPα of that of PTPα and and and on phosphorylation of PTPα to of the cells on to of the on and and the cells on of PTPα and the the tyrosine phosphorylation of PTPα that of the that was the PTPα upon the of PTPα to the of PTPα tyrosine phosphorylation We the upon integrin PTPα and Src and the of PTPα required were PTPα the of PTPα Tyr789 PTPα was and PTPα were of phosphorylation of PTPα that PTPα required to to PTPα of PTPα and cells tyrosine phosphorylation of cells and cells plated on of and phosphorylation of cells to the plated on that the phosphorylation of of PTPα the of Src to the of of integrin-stimulated phosphorylation at that Tyr789 was the PTPα upon integrin the of PTPα. and were from cells were the PTPα was PTPα were and plated on to PTPα tyrosine phosphorylation and cells PTPα was of cells cells that the PTPα at Tyr789 and that upon integrin SFKs PTPα that PTPα phosphorylation SFKs was PTPα was dephosphorylated when Src were and was rephosphorylated when the cells were plated on that Src PTPα the SFKs and PTPα phosphorylation was from the substratum and on PTPα phosphorylation cells Src and PTPα was dephosphorylated when the cells were and rephosphorylated when the cells were plated on on PTPα tyrosine phosphorylation were when from cells of were of of and that integrin-stimulated phosphorylation of PTPα at PTPα and to PTPα induces and the of the were PTPα and was and of PTPα detachment was and tyrosine phosphorylation of PTPα was the of and phosphorylation of at and and that required PTPα integrin-stimulated phosphorylation the required PTPα of and and PTPα phosphorylation when the were and on of PTPα phosphorylation the cells was to that the cells and that the tyrosine kinase PTPα phosphorylation and that PTPα phosphorylation of PTPα Tyr789 at early integrin required integrin-stimulated phosphorylation of at PTPα activates and PTPα was to the of PTPα to PTPα Tyr789 phosphorylation required integrin-stimulated and the tyrosine phosphorylation of cells of PTPα PTPα and tyrosine phosphorylation were of of PTPα cells PTPα was and tyrosine phosphorylation was cells cells and and on phosphorylation were to phosphorylation Src phosphorylation was Src the Src dephosphorylated at Src of phosphorylation and the of Src were Src was cells phosphorylation the of Src phosphorylation of Src at cells cells of the of phosphorylation of cells of PTPα of Src at phosphorylation of Src at and phosphorylation of at to the the of PTPα on Src phosphorylation and was to phosphorylation of cells Src and tyrosine phosphorylation to and to of α PTP PTPα Tyr789 required PTPα to phosphorylation of integrin of PTPα induces phosphorylation (Src and Src that required integrin-stimulated phosphorylation and of PTPα cells integrin-stimulated phosphorylation of at to that cells phosphorylation at that cells of and the kinase were upon of PTPα and that phosphorylation of PTPα at Tyr789 required early required to integrin-stimulated and tyrosine PTPα the of PTPα. PTPα Tyr789 cells on of and focal of PTPα the cells We the of PTPα and phosphorylation at required of integrin-stimulated cells were plated on and and the cells were and to and on was that cells PTPα on the substratum and of and the to cells and that was the the cells to on and the cells that of and was cells cells PTPα. on cells cells PTPα were and and focal cells and cells was at were cells PTPα cells and and of and focal adhesion of the cells PTPα to and the cells and and PTPα that the of PTPα and phosphorylation at Tyr789 integrin-stimulated and focal adhesion PTPα Tyr789 and integrin-stimulated that required cells to of cells that of PTPα of cells PTPα of cells the of to and focal adhesion PTPα PTPα was to of cells the cells that PTPα tyrosine phosphorylation to integrin PTPα dephosphorylated when cells and rephosphorylated when cells plated on fibronectin. phosphorylation at Tyr789 the of PTPα and Src PTPα and activates SFKs and of SFKs early integrin PTPα Src and and PTPα tyrosine and the autophosphorylation required that required the phosphorylation of PTPα at Tyr789 that integrin required PTPα phosphorylation that PTPα phosphorylation of the and required integrin-stimulated PTPα tyrosine phosphorylation phosphorylation and and PTPα tyrosine phosphorylation to that PTPα Tyr789 phosphorylation required was PTPα Src and of and and and to that phosphorylation of PTPα at Tyr789 to and cells was to to Src at of Src that of the PTPα to the that were of PTPα of the was to to to of Src at and of Src of Src was the Src to of the of Src and the of PTPα and Src to of Src at PTPα of PTPα was to α and that Src and Src that PTPα Tyr789 phosphorylation required integrin-stimulated to PTPα We that integrin that the PTPα of PTPα and Src the the of PTPα to SFKs to that the PTPα to ligand the integrin the Src the the Src of Src to the of the Src that of Src to Src integrin that and PTPα to promote and PTPα tyrosine PTPα Tyr789 phosphorylation required phosphorylation and tyrosine phosphorylation of the required integrin on and the of and of focal the of PTPα of the the to that tyrosine phosphorylation of PTPα required of PTPα that at integrin to and focal adhesion that PTPα of SFKs PTPα phosphorylation and the stimulation activates and to and the of and activates to promote and the of the of focal adhesion that and and focal adhesion cells that PTPα phosphorylation the of of of of PTPα the that PTPα the Tyr789 PTPα required PTPα to to focal of the focal adhesion to that from to the integrin the of PTPα and Src the that of of PTPα integrin PTPα early of the SFKs Src and phosphorylation and autophosphorylation at tyrosine phosphorylation of and PTPα early upon the of PTPα and to the of Src and We that phosphorylation of PTPα at Tyr789 Src required integrin the phosphorylation of PTPα required the of of to focal adhesion and We that PTPα tyrosine phosphorylation to integrin PTPα dephosphorylated when cells and rephosphorylated when cells plated on fibronectin. phosphorylation at Tyr789 the of PTPα and Src PTPα and activates SFKs and of SFKs early integrin PTPα Src and and PTPα tyrosine and the autophosphorylation required that required the phosphorylation of PTPα at Tyr789 that integrin required PTPα phosphorylation that PTPα phosphorylation of the and required integrin-stimulated PTPα tyrosine phosphorylation phosphorylation and and PTPα tyrosine phosphorylation to that PTPα Tyr789 phosphorylation required was PTPα Src and of and and and to that phosphorylation of PTPα at Tyr789 to and cells was to to Src at of Src that of the PTPα to the that were of PTPα of the was to to to of Src at and of Src of Src was the Src to of the of Src and the of PTPα and Src to of Src at PTPα of PTPα was to α and that Src and Src that PTPα Tyr789 phosphorylation required integrin-stimulated to PTPα We that integrin that the PTPα of PTPα and Src the the of PTPα to SFKs to that the PTPα to ligand the integrin the Src the the Src of Src to the of the Src that of Src to Src integrin that and PTPα to promote and PTPα tyrosine PTPα Tyr789 phosphorylation required phosphorylation and tyrosine phosphorylation of the required integrin on and the of and of focal the of PTPα of the the to that tyrosine phosphorylation of PTPα required of PTPα that at integrin to and focal adhesion that PTPα of SFKs PTPα phosphorylation and the stimulation activates and to and the of and activates to promote and the of the of focal adhesion that and and focal adhesion cells that PTPα phosphorylation the of of of of PTPα the that PTPα the Tyr789 PTPα required PTPα to to focal of the focal adhesion to that from to the integrin the of PTPα and Src the that of PTPα. of PTPα integrin PTPα early of the SFKs Src and phosphorylation and autophosphorylation at tyrosine phosphorylation of and PTPα early upon the of PTPα and to the of Src and We that phosphorylation of PTPα at Tyr789 Src required integrin the phosphorylation of PTPα required the of of to focal adhesion and We and of the and the of the

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.