First Report of Bacterial Wilt of Common Bean Caused <i>by Curtobacterium flaccumfaciens</i> in Western Canada
Notice bibliographique
Résumé
Bacterial wilt of common bean (Phaseolus vulgaris L.) caused by Curtobacterium flaccumfaciens pv. flaccumfaciens (Hedges) Collins & Jones (4) was found in 1947 in Ontario, Canada (3), but not in western Canada. Infected seeds exhibit yellow, orange, or purple discoloration (4). Examination of 36.7 kg of cull beans of crops grown in southern Alberta in 2001 obtained from a processing plant revealed 5.9% yellow and 0.014% orange seeds, each with wrinkled seed coats. Bacteria were isolated on potato dextrose agar. Three strains were identified using conventional tests (2), carbohydrate oxidation (GP Microplates, Biolog Inc., Hayward, CA), and cellular fatty acids (CFA) (MIDI, Inc., Newark, DE). Strains were gram-positive, motile, aerobic rods with yellow (YSB-1, YSB-2) or orange (OSB-3) colonies. Growth occurred at 27 and 37°C. The strains were positive for citrate utilization, catalase, hydrolysis of hippurate, and indoxyl acetate, and negative for urease, gelatin liquification, and oxidase. CFA profiles were ≈48% 15:0 anteiso, 37% 17:0 anteiso, 8% 16:0 iso, 3% 15:0 iso, and 3% 16:0; with17:1 anteiso A sometimes present at <2%. Acid production was weak from carbohydrates, but all oxidized many carbohydrates in the microplates. These results match C. flaccumfaciens pv. flaccumfaciens (2) in MIDI and Biolog databases. Strains were tested for pathogenicity using seed and pod inoculations. Seeds of great northern (‘US1140’) and navy (‘AC Skipper’) beans were soaked in bacterial suspension (1 to 3 × 10 8 CFU/ml) for 1 h, sown in Cornell Peatlite Mix (1) in Root Trainers (Spencer-Lemaire Industries, Edmonton, AB, Canada), incubated at 28°C (16-h day) and 22°C (8-h night), and examined for seedling wilt after 10 days. Seeds soaked in sterile distilled water served as controls. Testing was repeated once with 3 replicates per treatment and 10 seeds per replicate. Experiments were conducted using a complete randomization design. For pod inoculation, a suspension (0.1 ml) of each strain was injected into the midrib at the basal end of each young pod of ‘AC Skipper’. Pods inoculated with sterile distilled water, 0.1 ml per pod, were used as controls. After 21 days, pods were harvested and examined. Testing was repeated once with three plants per treatment and five pods per plant. Bacteria were reisolated from hypocotyls of wilted seedlings and diseased pods. Results of seed inoculations showed all strains were pathogenic to both cultivars. Wilt incidence was 38, 35, and 57% for strains YSB-1, YSB-2, and OSB-3, respectively, on ‘US1140’ and 44, 40, and 63% respectively, on ‘AC Skipper’. Results of pod inoculations showed 63% (YSB-1) and 55% (YSB-2) of seeds had wrinkled, yellow seed coats, and 72% (OSB-3) of seeds had wrinkled, orange seed coats. Control seedlings and seeds remained healthy. C. flaccumfaciens pv. flaccumfaciens was reisolated from wilted seedlings and seeds showing yellow or orange discoloration, but not from the controls. To our knowledge, this is the first report of bacterial wilt of bean caused by yellow and orange strains of C. flaccumfaciens pv. flaccumfaciens in western Canada. References: (1) J. W. Boodley and R. Sheldrake Jr. N.Y. State Coll. Agric. Life Sci. Inform. Bull. 43, 1977. (2) K. Komagata et al. Page 1313 in: Bergey's Manual of Systematic Bacteriology, Vol. 2, Williams and Wilkens, Baltimore, MD, 1986. (3) Z. A. Patrick, Can. J. Bot. 32:705, 1954. (4) A. W. Saettler. Bacterial wilt. Page 31 in: Compendium of Bean Diseases. R. Hall, ed. American Phytopathology Society, St. Paul, MN, 1994.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».