114 BOVINE EMBRYO-MATERNAL RECOGNITION MAY OCCUR EARLIER IN AI THAN IN EMBRYO TRANSFER (ET)
Notice bibliographique
Résumé
Embryo-maternal recognition is known to be mediated by the trophoblast and/or its secretory product during the preimplantation period. However, it was recently proposed that embryo-maternal recognition (along with the maternal immune system) could be activated by zona pellucida-degradation products including oligosaccharide chains from very early stage embryos (Fujiwara et al. 2009 J. Reprod. Immunol. 81, 1–8). If this is true, we predict that mammalian mothers recognise the presence of an embryo immediately after fertilization in vivo. The purpose of the present study was to compare the expressions of immune tolerance genes in bovine endometrial-luminal lymphoid (ELL) cells collected from animals after AI or embryo transfer (ET). ELL cells were collected from recipient cows inseminated with frozen–thawed semen on Day 0 (=oestrus, n = 8) and from cows that received a transferred embryo on Day 7 (n = 8) of the oestrous cycle. On Days 9 and 11, ELL cells ipsilateral to the corpus luteum were flushed non-surgically from four cows of each group using Ringer's solution (500 mL). To eliminate large cell clusters of endometrium and the embryo (blastocyst), the flushing medium was passed through an embryo collection filter (EmCon filter, 75 µm stainless steel screen). A single viable blastocyst was collected from each recipient cow. The recovered flushing medium containing ELL cells was centrifuged. The ELL cells were suspended in PBS and separated by Lympholyte-H (Cedarlane, Ontario, Canada) gradient centrifugation. Using RT-real-time PCR, transcripts for four immune tolerance genes in ELL cells from AI or ET examined were transforming growth factor-ß (TGF-ß), macrophage-colony stimulating factor (M-CSF), interleukin-10 (IL-10), and forkhead box P3 (Foxp3). Data were normalized to that of an endogenous control (glyceraldehyde-3-phosphate dehydrogenase) and analysed using ANOVA followed by Scheffe's test. Transcripts for TGF-ß, M-CSF, IL-10, and Foxp3 were detected in all RT-samples derived from ELL cells on Days 9 and 11 of the oestrous cycle. Transcript levels of TGF-ß, M-CSF and IL-10 in the AI group were the same as those of the ET group (P > 0.05), although the transcript level of Foxp3, a master regulator of the function of regulatory T-cells, in the AI group was significantly higher than that of the ET group on Days 9 and 11 (P < 0.05). Furthermore, the transcript level of Foxp3 in the ET group on Day 11 was significantly higher than that of the ET group on Day 9 (P < 0.05). These results indicate that the embryo-maternal recognition in AI occurs earlier than in ET, and suggest that cows recognise an embryo earlier than those generally believed.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».