DISCOVERY OF A NEW REG3?? HAMSTER GENE HOMOLOGOUS TO THE HUMAN REG3 GENE
Notice bibliographique
Résumé
For over 60 years, insulin injections have been the standard treatment for diabetes mellitus even though they cannot prevent the devastating complications associated with the disease. Another approach to diabetes therapy would be to propagate new β-cells in the pancreas that could compensate for any insulin deficit. A family of regenerating genes (Reg) has been identified to be strongly expressed during islet neogenesis. We previously found a type 3 Reg gene in hamsters, Islet Neogenesis Associated Protein (INGAP) and have recently shown that administration of a portion of this protein can reverse hyperglycemia in rodents. Since then, a mouse INGAP related protein (INGAPrP/Reg 3δ) has been discovered, as has the human Reg 3 gene, which is claimed to be the counterpart to INGAPrP. Validating human Reg 3 as the INGAP/INGAPrP homolog is difficult since the Reg family is underrepresented in humans and hamsters compared to mice and rats. Our aim was to find new members of the Reg gene family in hamsters by using a tissue culture that was known to constitutively express INGAP. Isolated hamster acinar cells were embedded into a collagen matrix and cultured in DMEM/F12 leading to the formation of duct-like structures (DLS). To amplify Reg genes by reverse transcriptase polymerase chain reaction (RT-PCR), we designed degenerate primers based on the conserved domains of the Reg protein family. PCR products were visualized on agarose gel and appropriately sized products were gel eluted, sequenced and searched against the GenBank database. The RACE system was performed to further sequence the mRNA ends outside the degenerate primer boundaries. When PCR reactions were carried out using hamster DLS cDNA and the degenerate Reg primers, it resulted in the amplification of a novel type 3 Reg gene. The translated protein sequence was found to have the highest similarity to the rat Reg3γ protein at 80% and the mouse Reg 3γ protein at 79% indicating that it was a novel hamster Reg 3γ gene. Compared to the other hamster Reg proteins, it was found to be 55% similar to INGAP and 48% similar to Reg 1. When the human Reg 3 protein sequence was evaluated, it was discovered that human Reg 3 has 69% homology to hamster Reg 3γ compared with only 54% homology to INGAP and only 50% to its reported counterpart, INGAPrP. The discovery of hamster Reg3γ provides strong evidence that human Reg 3 is not the human INGAP/INGAPrP homolog. Elucidating all the Reg family members, including the human INGAP homolog, could provide us with an appropriate means for promoting islet growth in diabetic individuals.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».