65 ASSESSMENT OF LIVE OFFSPRING SIRED BY A SOMATIC CELL NUCLEAR TRANSFER BULL, STARBUCK II
Notice bibliographique
Résumé
It appears that overt phenotypic abnormalities observed in some domestic animal clones are not transmitted to their progeny, suggesting that epigenetic and genetic alterations are reset in the germline of clones. Still, little is known about the normalcy and performance of the offspring from cloned animals. The current study monitored Holstein heifers sired by a cloned bull, Starbuck II, from weaning to puberty. Genomic stability was assessed by telomere length status and chromosomal assessments. Growth parameters, blood profiles and physical exams were performed and the results were compared to those for a group of control heifers with matched breed and ages born from normal cows located in the Ponsonby Research Station (Guelph, Ontario, Canada). Once circulating progesterone concentrations were determined to be >1 ng/mL, the heifers were monitored by ultrasound to investigate follicular dynamics. Statistical analysis was performed using descriptive statistics, paired t -test, and a Tukey-Kramer post-hoc test for multiple comparisons. Compared to controls during the observation period, progeny sired by the cloned bull did not show any significant ( P > 0.05) difference as in weight (mean ± SEM) (231.5 ± 29.4 vs. 237.6 ± 31.0 kg), length (160.6 ± 13.0 vs. 172.4 ± 2.7 cm), height (105.3 ± 3.72 vs. 95.5 ± 11.0 cm) and temperature (39.06 ± 0.01 vs. 39.16 ± 0.0°C). However, the cases displayed a relaxed behavior and were easier to handle than controls. This was reflected by significant differences in heart rates (83.3 ± 3.3 vs. 101.3 ± 2.6 bpm) ( P = 0.005) and respiratory rates (35.4 ± 2.7 vs. 46.7 ± 3.3 rpm) ( P = 0.04) for progeny and controls, respectively. Blood profiles were within normal ranges for both groups. External and internal genitalia were normal at palpation and ultrasound exam. Both groups began puberty at the expected age (10-12 months) and weight (318 to 365 kg). Follicular dynamics and progesterone concentrations corresponded to normal adult cows and were similar between groups after puberty. Telomere lengths of spermatozoa and blood samples from Starbuck II (22.23 kb and 14.19 kb, respectively) were not statistically different from normal telomere length profiles for non-cloned cattle. Telomere lengths of Starbuck II's progeny (22.52 ± 0.38 kb, n = 32) were no different compared to age-matched controls (22.20 ± 0.56 kb, n = 20). In addition, the progeny exhibited a normal karyotype in their peripheral blood leukocytes (83.14 ± 5.22% normal diploid cells). In summary, heifers sired by a cloned bull have normal chromosomal stability, growth, physical, hematological and reproductive parameters compared to normal heifers. Also, these heifers had a better response with lower stress to regular handling and restraining procedures. This work was funded by OCAG, OMAF, and CRC.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».