<i>Campylobacter coli</i>Outbreak in Men Who Have Sex with Men, Quebec, Canada, 2010–2011
Notice bibliographique
Résumé
During September 2010-November 2011, a cluster of erythromycin-susceptible, tetracycline-and ciprofloxacinresistant Campylobacter coli pulsovar 1 infections was documented, involving 10 case-patients, in Montreal, Quebec, Canada.The findings suggested sexual transmission of an enteric infection among men who have sex with men.C ampylobacter coli is the second most common species that causes human Campylobacter infections (1-3).Few studies have characterized the differences between the epidemiology and the disease of C. coli infections in comparison to C. jejuni subsp.jejuni infections (1-3).However, many studies have reported a higher macrolide resistance in C. coli than in C. jejuni (1-3).Few C. coli outbreaks have been reported to date (4,5). The StudyA retrospective analysis, including the period from January 1, 2010 through December 31, 2011, identified 43 laboratory-confirmed cases of C. coli infections reported to the Montreal Public Health Department; among them, 40 cases with antimicrobial drug susceptibility results were further analyzed.Telephone interviews with the case-patients were conducted by using a standardized questionnaire pertaining to symptomatology of the illness, treatment, exposures, sexual orientation (including practices), and HIV status.The questionnaire was mailed to persons who could not be contacted by phone.Hospital charts for 9 or the 10 outbreak case-patients were reviewed retrospectively.Statistical analyses, using Fisher exact test to calculate the possibilities, were conducted to test for differences in characteristics between case-patients infected with the outbreak etiologic agent, C. coli pulsovar 1, and those infected with nonoutbreak C. coli.In estimating the odds ratio from a 2 × 2 table that included a zero cell, 0.5 was added to the count in each cell.CIs were calculated by using Miettinen's test-based method.Statistical analyses were conducted using SPSS software (http://www-01.ibm.com/software/analytics/spss/products/statistics/).Phenotypic identification of Campylobacter isolates at the genus and species levels was confirmed by cpn60 gene sequencing at Laboratoire de Santé Publique du Québec.C. coli strains were identified by direct sequencing of PCRamplified partial cpn60 sequences as described by Hill et al. (6).DNA sequences were determined with an ABI 3100 sequencer using a BigDye sequencing kit (Applied Biosystems, Foster City, CA, USA).The sequences were subjected to a BLAST analysis and aligned with the ClustalW program.Phylogenetic analysis was performed using the Lasergene software V6.1 (DNAstar, Madison, WI, USA).Genetic relatedness was investigated by using pulsed-field gel electrophoresis with SmaI according to PulseNet Canada procedures.Salmonella enterica serotype Braenderup strain H9812 was used as the marker size in each gel (7).For analysis, band position tolerance and optimization values of 1% were used.Similarity coefficient was obtained with the unweighted pair-group method with arithmetic averages.For strains exhibiting similar patterns with SmaI, a second enzyme (KpnI) was used to confirm their pulsed-field gel electrophoresis pattern similarity.The PulseNet Canada SmaI and KpnI pattern designations for the C. coli pulsovar 1 isolate are CASAI.0160and CAKNI.0078,respectively.Antimicrobial drug susceptibility testing was determined by using the disk diffusion method for erythromycin, tetracycline, and ciprofloxacin (8) and the Etest (AB Biodisk, Solna, Sweden) method for all 12 agents tested (3).b-lactamase susceptibility was determined as reported (9).From September 2010 through November 2011, in Montreal, 10 men, 26-57 years of age, were found to be infected with an erythromycin-susceptible, tetracycline-and ciprofloxacin-resistant C. coli pulsovar 1; these men were defined as the outbreak-associated case-patients (Figure 1).An additional 5 women and 4 men were infected with an
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,003 | 0,001 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,001 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».