30 SCRIPTAID TREATMENT IMPROVES POST-IMPLANTATION DEVELOPMENT OF SHEEP CLONED EMBRYOS
Notice bibliographique
Résumé
Increased histone acetylation by exposure to inhibitors of deacetylase enzymes has been reported to improve development of embryos produced by somatic cell nuclear transfer (SCNT). However, the response to such treatment seems to vary according to the species, cell line, and type of inhibitor used. The main objective of this study was to evaluate if treatment with the histone deacetylase inhibitor Scriptaid could improve the development to term of sheep SCNT-embryos. The 2 fibroblast cell lines used in this study were obtained from skin biopsies collected from 2 adult rams of the Santa Ines breed. Oocytes were collected by laparoscopic ovum pickup (LOPU) from 30 crossbred sheep that were hormonally stimulated as described previously (Baldassarre et al. 2002 Theriogenology 57, 275). Oocyte maturation, cell transfer, fusion and activation, culture and transfer to recipients were conducted following procedures previously described (Baldassarre et al. 2003 Cloning Stem Cells 5, 279). Briefly, oocytes were matured in vitro for 24 h in TCM 199 supplemented with hormones and 10% fetal bovine serum, at 38.5°C in 5% CO2. Cells were transferred into enucleated oocytes, followed by electric fusion using a single DC pulse of 1.6 kV cm–1 for 70 µs. The reconstructed embryos were then activated using ionomycin (5 µM/5 min) followed by cycloheximide (10 µg mL–1) and cytochalasin B (7.5 µg mL–1) for 4 to 5 h and then cultured in mSOF media (control); while half of the reconstructed embryos were exposed to 500 nM Scriptaid for 10 to 12 h starting after ionomycin treatment. Subsequent to culture in mSOF ± Scriptaid as above, selected embryos were finally transferred into the oviducts of synchronized recipients within 24 h from fusion. Pregnancy was detected and monitored for the first 3 months by transrectal ultrasound scanning. A total of 258 oocytes were recovered (8.6/donor), of which 203 resulted in fused embryos after micromanipulation (79%) and 178 (69%) were selected for transfer into the oviducts of 18 synchronized recipients (avg. 10 embryos/recipient). Initial pregnancy was significantly higher in the Scriptaid group (40 v. 12.5%; P < 0.01). Interestingly, pregnancy was maintained through gestation Day 90 in the Scriptaid group, while the pregnant recipient carrying the control embryos lost her pregnancy by Day 60. All 4 pregnant recipients are due in early August. Our results are consistent with a previous report from (Zhao et al. 2010 Cel. Reprog. 12, 75) working with pig embryos and suggest that Scriptaid treatment can improve post-implantation development of SCNT sheep embryos. The results above will be further evaluated when data from births becomes available.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».