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Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers

2011· article· en· 883 citations· W2094334461 sur OpenAlex· 10.1038/nbt.2022

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Résumé

Pigeonpea is an important protein source in many developing countries, but limited genetic resources have constrained its improvement. The draft genome sequence of pigeonpea, the first for a nonindustrial crop and for a grain legume, should facilitate molecular breeding efforts to improve yields for subsistence farmers. Pigeonpea is an important legume food crop grown primarily by smallholder farmers in many semi-arid tropical regions of the world. We used the Illumina next-generation sequencing platform to generate 237.2 Gb of sequence, which along with Sanger-based bacterial artificial chromosome end sequences and a genetic map, we assembled into scaffolds representing 72.7% (605.78 Mb) of the 833.07 Mb pigeonpea genome. Genome analysis predicted 48,680 genes for pigeonpea and also showed the potential role that certain gene families, for example, drought tolerance–related genes, have played throughout the domestication of pigeonpea and the evolution of its ancestors. Although we found a few segmental duplication events, we did not observe the recent genome-wide duplication events observed in soybean. This reference genome sequence will facilitate the identification of the genetic basis of agronomically important traits, and accelerate the development of improved pigeonpea varieties that could improve food security in many developing countries.

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La notice

Revue
Nature Biotechnology
Thématique
Genetic and Environmental Crop Studies
Domaine
Agricultural and Biological Sciences
Établissements canadiens
Organismes subventionnaires
Consortium of International Agricultural Research CentersIndian Agricultural Research InstituteUniversity of OttawaNational Research CentreIndian Council of Agricultural ResearchNational Science Foundation
Mots-clés
CajanusLegumeBiologyCropAgronomySequence (biology)BiotechnologyGenetics
Résumé présent dans OpenAlex
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