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RETRACTED ARTICLE: Role of dopamine D2 receptors in ischemia/reperfusion induced apoptosis of cultured neonatal rat cardiomyocytes

2011· article· en· 96 citations· W2098406857 sur OpenAlex· 10.1186/1423-0127-18-18

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Résumé

BACKGROUND: Myocardial ischemia/reperfusion injury is the major cause of morbidity and mortality for cardiovascular diseases. Dopamine D2 receptors are expressed in cardiac tissues. However, the roles of dopamine D2 receptors in myocardial ischemia/reperfusion injury and cardiomyocyte apoptosis are unclear. Here we investigated the effects of both dopamine D2 receptors agonist (bromocriptine) and antagonist (haloperidol) on apoptosis of cultured neonatal rat ventricular myocytes induced by ischemia/reperfusion injury. METHODS: Myocardial ischemia/reperfusion injury was simulated by incubating primarily cultured neonatal rat cardiomyocytes in ischemic (hypoxic) buffer solution for 2 h. Thereafter, these cells were incubated for 24 h in normal culture medium. RESULTS: Treatment of the cardiomyocytes with 10 μM bromocriptine significantly decreased lactate dehydrogenase activity, increased superoxide dismutase activity, and decreased malondialdehyde content in the culture medium. Bromocriptine significantly inhibited the release of cytochrome c, accumulation of [Ca2+]i, and apoptosis induced by ischemia/reperfusion injury. Bromocriptine also down-regulated the expression of caspase-3 and -9, Fas and Fas ligand, and up-regulated Bcl-2 expression. In contrast, haloperidol (10 μM) had no significant effects on the apoptosis of cultured cardiomyocytes under the aforementioned conditions. CONCLUSIONS: These data suggest that activation of dopamine D2 receptors can inhibit apoptosis of cardiomyocytes encountered during ischemia/reperfusion damage through various pathways.

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La notice

Revue
Journal of Biomedical Science
Thématique
Cardiac Ischemia and Reperfusion
Domaine
Medicine
Établissements canadiens
University of SaskatchewanLakehead University
Organismes subventionnaires
National Natural Science Foundation of China
Mots-clés
BromocriptineReperfusion injuryIschemiaApoptosisReceptorEndocrinologyInternal medicineAgonistPharmacologyDopamine receptor D2Superoxide dismutaseLactate dehydrogenaseMedicineChemistryOxidative stressBiochemistryEnzymeHormone
Résumé présent dans OpenAlex
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