The Molecular Basis for the pH-activation Mechanism in the Channel-forming Bacterial Colicin E1

The in vitro activity of the channel-forming bacteriocins such as colicin E1 in model membranes requires the specific activation of the protein by an acidic environment in the presence of a membrane potential. Acid activation of the C-terminal domain results in the formation of an insertion-competent intermediate an of activation the of the of in the colicin E1 domain in membrane activation the of a The of by of the of results in a in the the of the the channel-forming in a of The in vitro activity of the channel-forming bacteriocins such as colicin E1 in model membranes requires the specific activation of the protein by an acidic environment in the presence of a membrane potential. Acid activation of the C-terminal domain results in the formation of an insertion-competent intermediate an of activation the of the of in the colicin E1 domain in membrane activation the of a The of by of the of results in a in the the of the the channel-forming in a of The a of by The of in a of the formation of in the of protein of the colicin activity protein membrane membrane protein membrane of E1 a of the channel-forming of by the the of the channel-forming the domain the of the domain the the of colicin E1 the membrane the the the domain a an insertion-competent the membrane of the an The the of in the of the of the of the an the membrane by the colicin E1 activity in the in the of The colicin E1 an by colicin E1 the of an acidic environment activation of the in The of an acidic environment in an activity in in colicin E1 an of protein in an the of of the the of a membrane of the of colicin E1 activation by in a the domain of of the the The the of an environment as a of the domain the the the colicin E1 domain a of the of a the of of the protein The of of the a of the the of the the the of of the colicin E1 the of the the a the of the activation of the domain by domain membrane membrane the of E1 E1 as protein the as of activity of colicin E1 a as of colicin E1 protein a of activity as the of the of The of the the as a of the of protein of in by a in as in a by in an by a as The the the as the The the as by of colicin E1 membrane the of by as a The the as The a in the a in the the the of the as the of by the in the of the by the of the as The protein in of as by in the The protein of The The the The of by the of the The the of the protein as the the of the the of the an of the in the a the as a the of the of formation by the protein in in the presence of a The in the a a an as of the the The the the the of of the of the the as in a of a of the the protein the The of the as the of The of the protein the of the protein the of of an a a a the as the the of the domain in colicin the of the a by the of of the domain the the of channel-forming by activity as the protein as a a of as the of the the domain by as in of in a the in the membrane activity of the of the the of the protein the of the membrane an an a the the of The of the the specific colicin E1 a the the of colicin E1 the membrane in the of the of the colicin E1 of the in of in the the of of the membrane a of the protein the of of the in the colicin E1 the of the an of in the by The C-terminal domain of colicin E1 a membrane a the the of the an in The in the of acidic the the of colicin The the the in of the membrane of colicin E1 the as by The in the protein the The the of of of of protein of a in membrane activity The the in the presence of the in the presence of The formation of a in the the the in the of the the of the in of the colicin E1 of a in of the in the in in a of the a as the protein the of the in the protein of of the protein a of as the of the as the as the of a the of the the of of of the of as as the protein in in the protein by the of in the in of the in in of in protein the of a of of the of protein in of the in of the in the of the of the the membrane a in the of channel-forming The the of the the in The membrane a the The of the in by in the membrane the The in as of the membrane the the the membrane the the membrane the the of the of the the of the by as in the the the in a in a in the the of the the a membrane the of the the protein the in vitro of colicin E1 protein the membrane as the of the by of the the of the membrane the protein The presence of the membrane of the the protein of the of as a of of the The of as a of of the of the by the of the protein the membrane The a of the the the the in the of protein the of a of the in a the of by a of a of in vitro activity of colicin E1 a of the of the in The activity as the protein as by an in the The protein a The of the the The of a the a activation The in The in the a in activity the the 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results of an of colicin an in of by the of of the the of colicin E1 of the a of the formation of such as as as specific of the in vitro acidic activation of the the of such an in the activation in the in of the the of of acidic the in vitro the formation of the membrane of colicin a environment the of membranes such as the the of colicin in vitro by environment the protein the of the membrane a of the of the protein the the by in of the protein a the in a intermediate of the of channel-forming the the of the the of the protein the of the membrane in the by of the membrane by the the C-terminal domain the in the membrane the of a of a a in of activation of the the the activation of the channel-forming of the the the of acidic in vitro activity of a of the environment by the as the protein the membrane of the of the acidic of the the of the membrane a the membrane as as an the model the of colicin E1 the of the the of colicin E1 the The of the protein the the the domain by the domain in the membrane as a the the of the the of the protein the the a of colicin formation the membrane of the the the the of the of protein as of of by the of by the of an of the protein such a the of the activation of in the of a of by the of acidic the of an of the acidic membranes the of specific as activation of the of the in the formation of the the a in an of the of of of the of of The a of by The of in a of the formation of in the of protein of the colicin activity protein membrane membrane protein membrane of E1 a of the channel-forming of by the the of the channel-forming the domain the of the domain the the of colicin E1 the membrane the the the domain a an insertion-competent the membrane of the an The the of in the of the of the of the an the membrane by the colicin E1 activity in the in the of The colicin E1 an by colicin E1 the of an acidic environment activation of the in The of an acidic environment in an activity in in colicin E1 an of protein in an the of of the the of a 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activation in the in of the the of of acidic the in vitro the formation of the membrane of colicin a environment the of membranes such as the the of colicin in vitro by environment the protein the of the membrane a of the of the protein the the by in of the protein a the in a intermediate of the of channel-forming the the of the the of the protein the of the membrane in the by of the membrane by the the C-terminal domain the in the membrane the of a of a a in of activation of the the the activation of the channel-forming of the the the of acidic in vitro activity of a of the environment by the as the protein the membrane of the of the acidic of the the of the membrane a the membrane as as an the model the of colicin E1 the of the the of colicin E1 the The of the protein the the the domain by the domain in the membrane as a the the of the the of the protein the the a of colicin formation the membrane of the the the the of the of protein as of of by the of by the of an of the protein 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channel-forming the the of the the of the protein the of the membrane in the by of the membrane by the the C-terminal domain the in the membrane the of a of a a in of activation of the the the activation of the channel-forming of the the the of acidic in vitro activity of a of the environment by the as the protein the membrane of the of the acidic of the the of the membrane a the membrane as as an the the the the of the of protein as of of by the of by the of an of the protein such a the of the activation of in the of a of by the of acidic the of an of the acidic membranes the of specific as activation of the of the in the formation of the the a in an of the of of of the of of the of of

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.