Susceptibility of Two Bighorn Sheep Cell Cultures to Selected Common Ruminant Viruses
Notice bibliographique
Résumé
Respiratory disease is a major factor in mortality in bighorn sheep populations in the western United States and Canada. The most common microorganisms isolated from the lungs of bighorn sheep that have died from respiratory disease are Pasteurella sp.9,12,13,16 Pasteurellae are opportunistic pathogens commonly found in the respiratory tract of both wild and domestic ruminants.8,20 These organisms often require other predisposing factors to establish disease in their host. There is evidence that viral infections predispose domestic livestock to develop pneumonic pasteurellosis;6,17,22 however, the role of Pasteurella in respiratory disease of bighorn sheep is not well understood. Antibody titers to respiratory syncytial virus (RSV), parainfluenza 3 (PI3) virus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus (BVDV) have been found in sera from healthy and clinically ill bighorn sheep.4,19 PI3 virus was isolated from a captive herd of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in Wyoming14 and later from free-ranging bighorns in Colorado.18 More recently, another respiratory virus, RSV, was isolated from a captive bighorn herd.5 Most laboratory cell lines currently used for virus isolation are of domestic animal origin. Viruses adapted to bighorn sheep may be more readily propagated in bighorn sheep cell cultures than in tissues from other animal species. The objectives of this study were to establish and evaluate cells of bighorn sheep origin for use in virus isolation. When 1 of 5 Rocky Mountain bighorn ewes captured for a study of lamb mortality9 died at 5 months gestation, the lamb was surgically removed for the production of tissue cultures. Kidneys were removed aseptically and placed in Hanks’ balanced salt solution. Cell suspensions were prepared from kidney cortical tissue by standard techniques.11 The prepared bighorn sheep kidney (BHSK) cells were suspended in growth medium (GM: minimal essential medium [MEM]a with 10% fetal bovine serum [FBS]b), seeded in 75cm2 tissue culture flasks, and incubated at 37 C in 5% CO2. The GM was exchanged frequently until confluent monolayers were established. Confluent monolayers were split 1:2 and passaged twice before freezing in GM with 10% dimethyl sulfoxidec in liquid nitrogen. Additionally, tissue fragments were removed from nasal turbinate bone and cartilage of the fetus. Tissue dissaggregation and cell collection, propagation, and preservation were carried out as above to produce turbinate cell cultures (BHST). The BHST and BHSK cells were passaged repeatedly to determine the number of viable passages. Coverslip cultures of both BHST and BHSK cells were examined for the presence of adventitious BVDV by direct fluorescence microscopy using fluorescein-conjugated antiserum to BVDVd and
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».