242. Turning of the Tide: Does Tidemark Advancement Perpetuate Osteoarthritis
Notice bibliographique
Résumé
Background: OA is a chronic debilitating condition marked by cartilage destruction and bone remodelling. Histopathologically OA is characterized by changes to the tidemark mineralization front, which marks the interface between the superficial viscoelastic hyaline layer and the deep calcified layer. Tidemark duplication (therefore, advancement) is a known histological feature of OA. This has been suggested to be a consequence of the reactivation of endochondral ossification leading to thickening of the calcified cartilage and neovascularization and neoinnervation of the cartilaginous cartilage potentially leading to mechanical pain. Methods: Femoral heads and tibial plateaus were taken from cadavers. Each femoral head was cut into four blocks exposing the articular surface, two superior to the fovea and two inferior. The tibial plateaus were cut into lateral or medial blocks. All blocks were demineralized by immersion in formal saline for a year, then embedded in wax and sliced into sections. These were divided into three even groups stained with toluidine blue, hematoxylin and eosin, and trichrome gomori. Each section was analysed by light microscopy and following the OARSI criteria set out by Pritzker et.al (2006) given a grade 0–6 based on changes to the matrix and/or cells. Student’s t-test explored associations between the presence of a dual tidemark and OARSI grade. Odds ratios were calculated for the occurrence of dual tidemarks by OARSI grade as independently defined by matrix and cells. Results: In total, 13 cadavers (8 male, 5 female, mean age 87) were used. Both femoral heads were removed from 5 cadavers, and one femoral head in two separate cadavers. The tibial plateau was removed from 6 different cadavers. 177 sections were produced (femoral heads left n = 72, right n = 72) (tibial plateaus right n = 22, left n = 11). A dual tidemark was associated with a higher OARSI grade than a single tidemark [mean (s.d.) 3.41 (1.59) vs 1.11 (1.04) respectively: P < 0.001]. A matrix with an OARSI score of ≥3 predicted a 96 fold increase in the association of a dual tidemark (P < 0.0001), whereas cellular changes resulting in an OARSI score ≥2 predicted a 17.7 fold increase in the occurrence of a dual tidemark (P < 0.001). Conclusion: We have demonstrated (for the first time) early changes in the matrix (OARSI ≥3) or chondrocytes (OARSI ≥2) strongly predict a dual tidemark. Pathologically, OA is regarded as a process that occurs from the wear and tear of articular surfaces. Our data suggest that tidemark advancement occurs at an early stage and may perpetuate further osteoarthritic change at the hyaline layer, thus mediating an alternative bottom-up mechanism. Further exploration of this may lead to novel systemic treatment strategies preventing tidemark advancement and thereby disease progression. Disclosure statement: The authors have declared no conflicts of interest.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».