Genetically Modified Human Derived Bone Marrow Cells for Posterolateral Lumbar Spine Fusion in Athymic Rats
Notice bibliographique
Résumé
In Brief Study Design. This experimental study consisted of four groups. In Group I, 8 animals were implanted with a collagen sponge containing 5 million human-derived bone marrow cells (HBMCs) infected with the BMP-2-containing adenovirus. In Group II, 5 animals were implanted with a collagen sponge containing 5 million HBMCs, which had been infected with an adenovirus containing the cDNA for the lacZ gene. In Group III, 5 animals were implanted with a collagen sponge containing 5 million uninfected HBMCs. In Group IV, 5 animals were implanted with a collagen sponge alone. All animals were killed at 12 weeks. Objectives. The purpose of this study was to assess the ability of HBMCs infected with a BMP-2 adenovirus to induce a posterolateral spine in an athymic rat model. Summary of Background Data. High pseudarthrosis rates after attempted spinal arthrodesis as well as the morbidity associated with iliac crest bone graft harvest make alternative bone graft materials desirable. Alternatives include allograft bone, demineralized bone matrices, and more recently, recombinant bone morphogenetic proteins. However, high doses of the recombinant protein are required, and a single dose of recombinant protein may not induce a sufficient osteoinductive response in all patients. An alternative strategy is to genetically manipulate cells to overexpress a bone morphogenetic protein. Methods. HBMCs grown in culture were infected with an adenovirus containing the cDNA for BMP-2 (AdBMP-2) or the gene for β-galactosidase (AdlacZ). Cells were implanted on the decorticated transverse processes of L4–L5 as previously described. Rats were assessed by radiographs at 4-week intervals and were killed at 12 weeks. After death, spines were explanted and assessed by manual palpation and histologic analysis. Results. Eight of eight rats implanted with AdBMP-2-infected HBMCs (Group I) were fused by radiographic evaluation and manual palpation at 12 weeks. Control groups consisted of: 5 rats implanted with the collagen sponge alone (Group II), 5 rats implanted with a collagen sponge and 5 million uninfected HBMCs (Group III), and 5 rats implanted with HBMCs infected with AdlacZ (Group IV). None of the rats in the control groups (0 of 15) developed a fusion at L4–L5. Conclusion. This study demonstrates that human-derived bone marrow cells can be infected with a BMP-2-containing adenovirus and produce sufficient bone in vivo to fuse the lumbar spine. This study assessed the ability of human-derived bone marrow cells infected with a bone morphogenetic protein 2 (BMP-2) containing adenovirus to induce spinal fusion in an athymic rat model. All (8 of 8) of the spines implanted with AdBMP-2-infected human derived bone marrow cells were assessed as fused at 12 weeks. None of the rats in the control groups developed a fusion at L4–L5. Human-derived bone marrow cells can successfully be infected with a BMP-2 containing adenovirus, produce bone in vivo, and fuse the lumbar spine in a nude rat model.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».