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PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes

2010· article· en· 2 574 citations· W2162792752 sur OpenAlex· 10.1093/bioinformatics/btq249

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Résumé

MOTIVATION: PSORTb has remained the most precise bacterial protein subcellular localization (SCL) predictor since it was first made available in 2003. However, the recall needs to be improved and no accurate SCL predictors yet make predictions for archaea, nor differentiate important localization subcategories, such as proteins targeted to a host cell or bacterial hyperstructures/organelles. Such improvements should preferably be encompassed in a freely available web-based predictor that can also be used as a standalone program. RESULTS: We developed PSORTb version 3.0 with improved recall, higher proteome-scale prediction coverage, and new refined localization subcategories. It is the first SCL predictor specifically geared for all prokaryotes, including archaea and bacteria with atypical membrane/cell wall topologies. It features an improved standalone program, with a new batch results delivery system complementing its web interface. We evaluated the most accurate SCL predictors using 5-fold cross validation plus we performed an independent proteomics analysis, showing that PSORTb 3.0 is the most accurate but can benefit from being complemented by Proteome Analyst predictions. AVAILABILITY: http://www.psort.org/psortb (download open source software or use the web interface). CONTACT: psort-mail@sfu.ca SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

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La notice

Revue
Bioinformatics
Thématique
Machine Learning in Bioinformatics
Domaine
Biochemistry, Genetics and Molecular Biology
Établissements canadiens
Simon Fraser UniversityUniversity of British Columbia
Organismes subventionnaires
Natural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchSimon Fraser UniversityMichael Smith Health Research BCCystic Fibrosis Foundation
Mots-clés
ProteomeProtein subcellular localization predictionArchaeaComputer scienceSubcellular localizationPrecision and recallComputational biologyInterface (matter)ProteomicsSoftwareMetagenomicsBiologyBioinformaticsData miningArtificial intelligenceBacteriaCytoplasmGeneticsGeneProgramming language
Résumé présent dans OpenAlex
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