Differential expression of dual <i>Bt</i> genes in transgene poplar Juba (<i>Populus deltoides</i> cv. ‘Juba’) transformed by two different transformation vectors
Notice bibliographique
Résumé
To increase the expression efficiency of the Bt gene in transgenic poplar and expand the insect resistance spectrum, Cry1Ac and Cry3A were simultaneously constructed in two different plant transformation vectors. Two Bt genes on vector p71A68Y71 were separately driven by promoters CAMV35S and CoYMV, and a matrix attachment region (MAR) sequence was added to both sides; in contrast, two Bt genes on vector p05A68A71 were driven by the promoter CAMV35S without a MAR sequence structure. With the agrobacterium mediation method, two vectors transformed the high-quality hybrid clone poplar Juba of Populus deltoides (W. Bartram ex Marshall) and thus produced four transgenic resistant lines. Polymerase chain reaction (PCR) showed that the targeted genes were integrated into the poplar Juba genome. Fluorescence quantitative PCR and enzyme-linked immunosorbent assay techniques were used to detect the transcription abundance and toxic protein expression of the Bt gene in the leaves of eight transgenic leaves. Results show the significant difference in transcription abundance of the two Bt genes between different lines. The transcription abundance of the gene Cry1Ac of the K series lines from vector p71A68Y71 was significantly higher than that of the H series lines from the other vector; however, no significant difference in the transcription abundance of gene Cry3A between the two vectors was observed. The transcription abundance of gene Cry3A was higher than that of gene Cry1Ac. Significant differences in the toxic expression of the two Bt genes existed among the lines but not in the H and K series lines. The Cry3A toxic protein content was much higher than the Cry1Ac toxic protein content in all lines, and the expression of the latter at 0.13 ng·g−1 to 0.69 ng·g−1 was extremely low. The insect-resistant effect of the transgenic lines to lepidoptera pests such as the Hyphantria cunea (Drury) larva was unremarkable, and no significant difference was found in different lines and vectors. The lines strongly resisted coleoptera pests, e.g., Plagiodera versicolora (Laicharting), and their insect-resistant effect was significantly higher than that of the highly resistant trans-Cry3A poplar line CC84. Significant differences were found between different lines, but no significant difference was found between the K and H series lines, which were from different vectors. Trangenic lines H4 and K1 had a higher transcription abundance, toxic protein expression, and lethal effect on P. versicolora than the other lines.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».