Notice bibliographique
Résumé
Microbial biofilms located on the lumen surface of dental unit water lines have resulted in the persistent and widespread microbial contamination of the outflowing water. The aims of this study were to 1) evaluate the water quality delivered by dental units at the University of British Columbia 2) evaluate the effect of plating and incubation conditions on heterotrophic plate counts and 3) evaluate the effectiveness of marketed products in reducing microbial counts in water used for nonsurgical and surgical dental procedures. Over a 12 week period, water samples were collected weekly from the air/water lines, highspeed lines and sinks (controls) of 8 randomly chosen dental units used for nonsurgical dental procedures. Following serial dilutions, samples were plated on R 2A agar using the spread plate technique, and incubated aerobically at 35°C for 7 days. Individual colonies that were isolated were characterized based on colony morphology, Gram stain and oxidase test results. Representative samples of dental tubing water lines were subsequently processed for scanning electron microscopy (SEM) and evaluated for the presence of biofilm. In order to determine the effect of culture conditions on heterotrophic plate counts, different culture media, plating techniques, incubation time and temperature and chlorine neutralization were evaluated. In addition, devices such as in-line filters, independent water reservoirs, chemical disinfection and fully autoclavable systems were evaluated for their effectiveness at reducing the heterotrophic plate counts. Scanning electron microscopy was used to visualize the surface of in-line filters after several minutes of use, and to determine if 0.12% chlorhexidine injected in post filter tubing was able to prevent biofilm formation. The results of these studies revealed that while all water samples collected from the cold water sink taps met the standards for potable water, water collected from the warm water sink taps, the air/water lines and the highspeed lines revealed extremely high heterotrophic plate counts and failed to meet the potable water standards in all but two instances. Although the plating techniques analyzed did not differ significantly, the incubation conditions had very significant effects on heterotrophic plate counts and dramatically affected the number of units that failed to meet potable water standards. The evaluation of products which are currently being marketed as effective ways to improve dental unit water line quality appear promising, however, aspects of their design may still leave concerns for some practitioners.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».