Abstract P2-09-16: Effect of Continuous Statistically Standardized qPCR and IHC ER and PR on Disease-Free Survival in NCIC CTG MA.12
Notice bibliographique
Résumé
Abstract Background: Inter-laboratory comparability of ER and PR assay results has been problematic for decades. Statistical standardization has been proposed as an adjunct following good laboratory quality control. We demonstrate this process for ER and PR centrally assessed by qPCR and IHC. Methods: NCIC CTG MA.12 is a placebo-controlled trial of tamoxifen therapy following adjuvant chemotherapy in premenopausal women with early breast cancer. 672 women were accrued, 505 of whom had locally determined ER and/or PR positive tumours. ER was centrally assessed by qPCR and IHC for respectively 385 and 392 patients, and by PR, for 389 and 376. The effects on DFS of continuous 1) log2 (qPCR) and 2) ln (IHC % positive stain) in statistically standardized units were investigated for all women with all lab results, and in the subgroup with IHC ER+ and/or PR+ tumours. DFS is defined as the time from randomization to recurrence or death. Multivariate examinations were with adjusted Cox regression, with adjustment by stratification factors nodal status and chemotherapy type; ER and PR had forced inclusion in models, while trial therapy and baseline patient characteristics were added in step-wise mode (p<=0.05). Unvariate tests were with adjusted log-rank statistic and Kaplan-Meier plots for ER and PR (ER and PR 0; standardized cut-points for ER and PR with positive stain of <= 1.0; > 1.0, <= 0.0; > 0.0, <= 1.0; >1.0). Results: Median follow-up for the MA.12 trial was 9.7 years. Univariately, qPCR ER was not associated with DFS (p=0.19), while both qPCR PR (p=0.09) and IHC ER (p=0.08) had weak evidence of association, and IHC PR had p=0.04. Multivariately, patients randomized to tamoxifen had significantly better DFS (p=0.002-0.005) in all patients assessed and in those with ER and/or PR > 0; ER and PR by qPCR and IHC were not associated multivariately with DFS (P>0.05). ER and PR were not associated with DFS (P>0.05) for those who received tamoxifen, while qPCR ER and/or PR, and IHC ER were inconsistently associated with DFS for those on the placebo arm. Conclusions: In this subgroup of centrally assessed MA.12 patients, trial therapy was significantly associated with DFS. Similar non-significant multivariate results were obtained multivariately with statistically standardized, continuous qPCR and IHC ER and PR. We plan to further investigate adjunctive statistical standardization in larger NCIC CTG trials. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P2-09-16.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,002 | 0,002 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».