Abstract 777: Antitumor role of early infiltrating interferon-gamma producing NKT cells in murine malignant mesothelioma
Notice bibliographique
Résumé
Abstract Introduction: NKT cells can provide adjuvant activity against cancer by producing large amounts of IFN-gamma which activate other immune cells, and orchestrate protective anti-tumor immunity. Recently, induction of the NKT cell-dependent anti-tumor immune response using its ligand alpha-galactosylceramide has been attempted in several tumor types. However, the role of NKT cells in the tumor microenvironment has not yet been fully addressed. Our aim is to elucidate the role of NKT cells in the thoracic cavity by using a murine malignant mesothelioma model. Methods: Half a million of AB12 murine malignant mesothelioma cells were injected into the right pleural cavity of female Balb/c mice for tumor development. The control mice were injected with the same amount of PBS. In this model, thoracic cavity was filled with tumors and pleural effusion by day 14. On days 0 (before tumor cell injection), 3, 6, 10 and 14 after tumor cell injection, mice were sacrificed and the pleural effusion and tumors were collected. Lymphocytes were isolated by performing a Ficoll gradient centrifugation. Cell phenotype was identified by cell surface marker staining and cytokine expression was analyzed by intracellular cytokine staining using flow cytometry. Cytokine concentrations in the pleural effusion were measured by ELISA. Resluts: The absolute number of CD3 positive cells in the pleural cavity did not increase between day 0 (4.7±0.9 x105), day 3 (5.5±0.7 x105) and day 6 (7.1x±1.6 x105) but did start to increase from day 10 (1.2±0.2 x106) after tumor cell injection. In the control mice, the absolute number of CD3 positive cells was not different throughout the experiment. Although the absolute number of CD8 positive cells and CD4 positive cells were not different between day 0 (CD8: 1.5±0.2 x105, CD4: 2.8±0.2 x105, respectively), day 3 (CD8: 1.8±0.2 x105, CD4: 2.6±0.2 x105, respectively) and day 6 (CD8: 1.9±0.4 x105, CD4: 4.3±0.2 x105, respectively) after tumor cell injection, the absolute number of NKT cells (CD3 and DX5 double positive cells) increased dramatically at relatively earlier phase from 1.6±0.1 x104 on day 0, to 6.4±0.3 x104 on day 3 and 12.1±1.0 x104 on day 14. Intracellular IFN-gamma staining of NKT and CD8 T cells showed that the ratio of IFN-gamma positive NKT to whole NKT cells started increasing from day 3 (day0: 7.0±1.3%, day3: 31±3%, respectively) and peaked on day 6 (59±11%) whereas the ratio of IFN-gamma positive to whole CD8 T cells started increasing from day 6 (day0: 12.1±3.5%, day6: 36±6.5%) and peaked on day 10 (39±3.4%). Conclusion: These results indicate that the NKT cells are recruited to the pleural cavity at a relatively early phase of tumor formation and could potentially affect the subsequent CD8 T cell response by the production of IFN-gamma. Further experiments are on going to see the role of NKT cells and to test the impact of chemotherapy on these cells in this model. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 777. doi:10.1158/1538-7445.AM2011-777
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».