Notice bibliographique
Résumé
Gordon Keller is Director of the McEwen Centre for Regenerative Medicine at the University Health Network in Toronto, Canada. His research applies concepts from developmental biology to the investigation of the lineage-specific differentiation of mouse and human embryonic stem (ES) cells. He became an Editor of Development in 2011, and recently we asked him a few questions to find out more about him and his research.Who or what inspired you to study science?I was always curious, and I found a scientific career to be one that allowed me to explore my curiosity.What sparked your interest to work on the directed differentiation of stem cells?That was a seminar by Rolf Kemler in 1984. I was in the Basel Institute for Immunology – I had arrived there about a year earlier – and Rolf came to the institute and showed us these beautiful, huge cystic embryoid bodies, in which you could see blood and vascular structures and beating heart cells. Knowing that you could make that from an ES cell piqued my interest and I decided to pursue research in this topic.What made you return to Canada after having worked in Switzerland and the USA?There were several things. First, there was an opportunity here to direct the McEwen Centre for Regenerative Medicine. Canada, and Toronto in particular, has a very strong scientific community but also a very strong stem cell biology community. And I am Canadian, and felt it would be a wonderful opportunity to return home and spend part of my career here.What has been the biggest surprise that you have come across in your research?I don’t know whether you would call it a surprise, but I have been amazed at the speed at which stem cell research has progressed. We have worked for years at differentiating mouse ES cells, and, although people were interested, it was always somewhat on the back burner. Then the discovery of human ES cells and induced pluripotent stem (iPS) cells transformed the field, and the kind of work we do has now become more mainstream. In a nutshell, I don’t know if I have been surprised by any particular finding so much, but what I find most remarkable is the evolution of the field and seeing it change almost on a weekly basis.Given these ongoing changes, where do you see the field move next?I think the biggest challenge that we have is to find a way to get the cells that we make in a dish to integrate into adult tissue and function. We are certainly making components of human tissues and organs, but to date there is not much evidence yet that they are functional, so I think the next hurdle – the big challenge before we can really make an argument that these are clinically relevant cells – is to find out whether in vitro differentiated cells can integrate into adult organ function.How does developmental biology inform in vitro differentiation?Developmental biology is the basis of all we do. For the last eight years, we have looked closely at concepts from developmental biology; for example, the pathways that control lineage specification in the early embryo. We initially applied these concepts to mouse ES cells, and more recently to human ES cells. Using knowledge from developmental biology has provided us with a very informed way to develop strategies and protocols that are both robust and efficient.What is the role of Development within your field?Many of the key papers that we look at to inform our work have been published in Development, and we have published a lot of our own ES cell work in the journal as well. At times, publishing our work has been challenging, I must say, because when we started it was a new system and a lot of people didn’t believe that cells in a dish could recapitulate development. But Development was very supportive and allowed us an avenue to publish our research.Is there a particular type of in vitro differentiation paper that you would encourage people to submit to Development?Absolutely. I would like to see ES cell differentiation papers coming to Development. This could include papers that use the system to study aspects of development that are very difficult to study in an embryo, and there are many examples of that. As we are starting to move from animal models towards human biology, ES cell differentiation is going to be the model for human developmental biology, and I would be delighted if the journal could stake a claim to human developmental biology.If you were not a scientist, what career would you have chosen?I have no idea. In fact I’m not sure that I had a priority to start with. I didn’t grow up saying ‘I want to be a scientist’, but rather I followed a path where my thoughts were along the lines of ‘I find this interesting, I’ll pursue it somewhat more’.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».