Characterization of Endophytic Pseudomonas Sp. 16 Strain and Its Use for the Enhancement of Phytoextraction
Notice bibliographique
Résumé
Extended Abstract Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies has attracted the most attention for the bioremediation of soils polluted with these contaminants. The success of metal extraction depends on many factors, but the most important are a plant’s ability to uptake and translocate metals to its stems and leaves, metal bioavailability and soil type. The results of many studies have shown that the efficiency of heavy metal phytoextraction may be supported by metal-resistant bacteria that belong to the plant growth-promoting bacteria (PGPB) group. Among them plant growth-promoting endophytes (PGPE) may confer plants with a higher tolerance to heavy metal stress and may stimulate the growth of the host plant through several mechanisms. The aim of this study was to estimate the potential of a metal-resistant strain of Pseudomonas sp. 16, isolated from Silene vulgaris tissues, to enhance Zn, Cd and Cu uptake by white mustard under laboratory conditions. Moreover, its potential to promote the growth of plants was also determined. Additionally, the ability of spontaneous rifampicin-resistant mutant of tested strain to colonize the internal tissues of Sinapis alba and its survival after introduction into soil were also determined. The strain showed the high resistance to Cd, Zn and Cu and produced large and clear orange haloes around the colonies grown on CAS agar, which indicates siderophore production. The activity of ACCD was 2.3 μmol α-ketobutyrate mg−1 h−1. The strain also produced IAA at a concentration of 6.96 ± 2.41 μg IAA mL−1 and was able to solubilize Ca3(PO4)2 in an NBRIP medium. The halo size was 13.67 ± 1.15 mm. The presence of the color orange on the paper filter placed on agar plates supplemented with glycine demonstrated the ability of the tested strain to release hydrocyanic acid. Moreover, this strain exhibited extracellular cellulase activity and was capable of motility and these features facilitated the plant colonization by bacteria. Plant growth-promoting features of tested strain caused higher biomass and heavy metal accumulation by white mustard growing in soil inoculated with suspension of tested strain, as compared with control plants growing in soil treated with suspension of thermal inactivated strain. A metal-tolerant Pseudomonas sp. 16 strain, due to the activity of several mechanisms that are considered to be important for plant growth-promotion has the potential to enhance the phytoextraction of heavy metals from contaminated soil. Additionally, tested strain showed a high survival rate after introduction into the soil and was able to colonize the internal tissues of S. alba. These features indicate that Pseudomonas sp. 16 strain in combination with S. alba can be regarded as an effective tool for the phytoextraction of heavy metals.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».