Stage-Specific Gene Expression Profile Differences During Early Porcine Embryonic Development.
Notice bibliographique
Résumé
During the pre-implantation period of embryonic development, the porcine embryo exhibits dramatic morphological changes associated with key developmental events such as compaction, differentiation, blastocyst formation and hatching. However, the molecular mechanisms underlying these events are mostly unknown. To better elucidate these mechanisms at the gene expression level, a comparative transcriptomic analysis of in vivo-derived porcine embryos representing 4 different developmental stages (4-cell, morula (MOR), expanded blastocyst (EXB) and hatched blastocyst (HB)) was performed with a custom designed porcine embryo-specific microarray (http://embryogene.ca/). In this study, total RNA samples were extracted from pools of 5 embryos of each embryonic stage and 3 biological replicates from each stage were utilized. They were compared to a reference sample consisting of pooled total RNA from 8 different porcine embryonic stages (GV, MII, 2-cell, 4-cell, 8-cell, MOR, EXB and HB). Microarray data were analyzed with the Flexarray1.6.1 software. The PANTHER (http://www.pantherdb.org/) and Ingenuity Pathway analysis software were used for the biological process and pathway analysis. The results of this comparative analysis across the four embryonic stages revealed 1517, 1286, 1286 and 1813 differentially expressed genes across stages from 4-cell, MOR, EXB and HB stages respectively. Further analysis was conducted to identify genes that were differentially expressed uniquely in one of the four embryonic stages evaluated and this revealed that 768, 228, 133 and 412 genes were specifically differentially expressed in the 4-cell, MOR, EXB, and HB embryos respectively. Among these stage-specific differentially expressed genes, 46, 17, 17 and 50 were found to be related to specific developmental processes. These genes were primarily involved with biological processes such as embryonic development, pattern specification processes (such as anterior/posterior and dorsal/ventral axis specification) and system development. It was also determined that several genes involved in homeostatic processes were uniquely differentially expressed in the 4-cell (ATP1A1, PTPN23), MOR (NUCB2) and HB embryos (ATP8B1, PTPRK, AGR2 and PSAP). Further analysis identified 10 genes related to homeostatic processes that showed differential expression across the four evaluated stages, 5 of them (AGR2, ATP1A1, ATP2B1, ATP8B1 and NUCB2) were related to cellular calcium ion homeostasis, 3 (PTPN23, PTPRK and SOCS5) were related to cellular glucose homeostasis and 2 (AGR2 and PSAP) were related to the regulation of liquid surface tension. In comparison with the 4-cell stage, 5 genes (AGR2, ATP1A1, ATP2B1, ATP8B1 and SOCS5) were down-regulated in MOR stage embryos and continued to be down-regulated at the EXB and HB stages. Two other genes (ATP12A, NUCB2) were up-regulated in MOR and then down-regulated in the EXB. Fluid accumulation in the embryo, caused by homeostatic mechanism, is critical for a morula to develop to a blastocyst and forth hatching process of that blastocyst. The regulation of homeostasis-related genes in morula and expanded blastocyst stage embryos may play an important role during blastocyst formation and hatching. This study has identified potential gene markers of early embryonic quality in pigs and increases our understanding of the molecular mechanisms involved in early embryonic development.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».