Src Is a Signaling Molecule in Ouabain-Na+K+-ATPase-Stimulated Signaling Pathway During Bull Sperm Capacitation.
Notice bibliographique
Résumé
Na+K+-ATPase acts as a signaling molecule when ouabain binds, inducing dose-dependent protein tyrosine phosphorylation (p-tyr) during sperm capacitation. Src is present in head membranes of bull sperm and its expression alters during ouabain-induced p-tyr, so this study investigated Src's role in capacitation. Fresh bull sperm (n=3-6 ejaculates per trial) were pre-incubated with or without Src inhibitors herbimycin A (inhibits kinase) or SU6656 (prevents ATP binding to Src), followed by incubation in the presence or absence of ouabain or heparin. Extracted proteins were assessed by western blotting, band intensity measured, and quantities of each protein band compared by ANOVA. Both protein p-tyr and LPC-induced acrosome reaction increased (p<0.05) upon exposure of sperm to ouabain (242, 176, 81, 49 kDa) or heparin (176kDa). Inhibition of Src activity by herbimycin or SU6656 antagonized (P<0.05) various ouabain- and heparin-induced p-tyr, and herbimycin inhibited the ouabain-induced acrosome reaction, confirming Src's integral role in capacitation. Herbimycin actually reduced some Src itself (p<0.05) during ouabain- or heparin-induced capacitation incubation. However, since Src was activated by auto-phosphorylation in the presence of ouabain or heparin, it was of greater interest that herbimycin significantly reduced ouabain-enhanced Src autophosphorylation of the 59kDa phospho-Src and the other ouabain- (166-31kDa) and heparin-induced phospho-Src (130, 40kDa) (P<0.05) implying that those are coupling proteins or substrates of Src. Src can also be activated by serine phosphorylation (p-ser) and both ouabain and heparin significantly stimulated p-ser of various sperm proteins (254-18kDa). SU6656 inhibited ouabain-induced p-ser at 40 and 32kDa, but heparin overcame this inhibition, implying that the Na+K+-ATPase pathway requires ATP binding to Src. In contrast, heparin is less ATP sensitive, perhaps primarily stimulating the cAMP/PKA pathway. Ouabain appears to induce capacitation through the ERK pathway, since phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) during capacitation were ouabain-sensitive. SU6656 antagonized ouabain-enhanced phosphorylation of ERK2, and other ERKs (39-17kDa) at 2h and herbimycin inhibited ERK1 in control sperm incubated for 5h. Heparin overcame the Src inhibitors' effect on ERKs possibly through the heparin-induced cAMP/PKA pathway. In summary, this study demonstrates Src's activation and involvement in tyrosine phosphorylation-dependent signaling through involving and activating ERK1/2 (potentially ERK2) in ouabain-induced bull sperm capacitation or acrosome reaction. The ouabain-sensitive Na+K+-ATPase signaling cascade is unique and distinct from that of heparin, but this novel research does identify for first time that Src cross talks with the heparin signaling pathway to induce capacitation. (poster)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».