The whole genome sequencing of Acinetobacter-calcoaceticus-baumannii complex strains involved in suspected outbreak in an Intensive Care Unit of a pediatric hospital
Notice bibliographique
Résumé
Background: To analyze the genetic characteristics of Acinetobacter-calcoaceticus-baumannii complex strains isolated in suspected outbreak in a Intensive Care Unit of a pediatric hospital in order to promptly stop the dissemination of this dangerous strain.Methods: This study described the use of whole genome obtained by Next Generation Sequencing to define the clonality of 13 Acinetobacter-calcoaceticus-baumannii complex strains and to study their Resistoma. This was required because Acinetobacter baumannii is known to be resistant to desiccation and to disinfectants and is difficult to treat and eradicate. Thus, this microorganism is a major problem that demands a rigorous outbreak monitoring program to prevent and control the spread of the dangerous strain.Results: The first result of our analysis has been to describe precisely the characteristics of isolates involved in the nosocomial infection, reduce the dimension of the more problematic isolates sustaining the outbreak and promptly facilitate the control of the strains diffusion. Indeed, our study indicated that among the 13 Acinetobacter baumannii-calcoacteticus complex strains, identified by biochemical and mass-spectrometry assays, 7 were Acinetobacter baumannii, 5 were Acinetobacter calcoaceticus, and one was Acinetobacter haemolyticus. The analysis of clonality of Acinetobacter baumannii indicated that three strains of ST744 were identical for > 99.8% among them and thus have sustained an outbreak in Intensive Care Unit. All personnel and possible environment samples were also monitored and all the procedures aimed to the prevention and control of Hospital Acquired Infections (HAI), have been strictly enforced by the Hospital Infection Control Committee. This gave the possibility to trace a strain from an environmental contamination characterized by high degree of clonality with the one isolated from a patient. On the contrary Acinetobacter calcoaceticus strains were different from each other and thus they were not responsible for any outbreak. The Resistoma analysis indicated a correspondence between phenotypic and genotypic characteristics of analyzed strains.Conclusions: Next Generation Sequencing is an appropriate technique to trace the circulation of dangerous strains sustaining nosocomial infections. The combination of the high resolution genotyping together with the definition of the antibiotic genetic determinants and the precise species identification make this technique a useful tool employed as standard practice to control HAI.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».