High Oct-2 Protein Expression Is Associated with Decreased Overall Survival, While High Bob-1 Protein Expression Is Associated with Improved Overall Survival in Myeloma Patients.
Notice bibliographique
Résumé
Abstract Abstract 2822 Poster Board II-798 Background: Oct-2 and Bob1 are B-lymphoid lineage specific transcription factors which drive the expression of genes involved in B-lymphocyte and plasma cell development and differentiation, including the immunoglobulin heavy chain (IgH) genes and many others. It is also thought that these transcription factors drive the overexpression of oncogenes involved in IgH translocations through upregulation of the IgH enhancers. We sought to evaluate the expression of Oct-2 and Bob1 in multiple myeloma, a malignancy of the B cell/plasmacyte lineage, in order to determine their potential biological significance as determinants of disease behaviour/prognosis and as potential therapeutic targets. Methods: A tissue microarray containing triplicate samples from 220 myeloma patient samples was generated. Samples were collected at the time of diagnosis between 1993 and 2008. To measure Oct-2 and Bob-1 protein expression in the CD138+ plasma cells of each sample, fluorescent immunohistochemistry was used to stain the samples and the HistoRx PM-2000 platformTM was used to generate an AQUA score for each patient. Oct-2 AQUA scores were available for 169 patient samples and Bob-1 AQUA scores were available for 169 patient samples. The analysis was repeated using conventional immunohistochemistry and visual scoring of staining intensity. Results: The Oct-2 and Bob-1 AQUA scores were continuously distributed over the patient cohort; thus, subsequent analyses were done by dividing the patient cohort by the median Oct-2 or Bob-1 AQUA score and comparing the group with high AQUA scores to the group with low AQUA scores. The patient group with high Oct-2 AQUA scores had an OS of 52.8 months compared to 73.1 months in the group with low Oct-2 AQUA scores (hazard ratio [HR]=1.74, 95% confidence interval [CI]=1.11-2.73, p=0.0164). The patient group with high Bob-1 AQUA scores had an OS of 70.5 months compared with 38.3 months in the group with low Bob-1 AQUA scores (HR=0.46, 95% CI=0.29-0.71, p=0.0008). In a Cox regression proportional hazards model including autologous stem cell transplant (ASCT) status, serum albumin, serum β2-microglobulin and Oct-2 or Bob-1 AQUA scores, only ASCT status was independently predictive of OS. The patient group with low Oct-2 AQUA scores had a significantly higher proportion of ASCT recipients than the group with high Oct-2 AQUA scores. Conversely, the patient group with high Bob-1 AQUA scores had a significantly higher proportion of ASCT recipients than the group with low Bob-1 AQUA scores. Results were similar but not statistically significant when analyzing the TMA with conventional immunohistochemistry and visual scoring of staining intensity. Discussion: In this retrospective study high Oct-2 expression was found to be associated with decreased OS, while high Bob-1 protein expression was associated with increased OS. These results suggest that Oct-2 is a negative prognostic factor in myeloma, while Bob-1 is a favorable prognostic factor, and demonstrates the applicability of the HistoRx platform to myeloma bone marrow TMA analysis. Since this was a small, exploratory study these results must be interpreted with caution and must be confirmed in a larger, prospective analysis. Disclosures: No relevant conflicts of interest to declare.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,003 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».