Abstract 3110: Urinary miR-1825 and miR-484: An oncogene and a tumor- suppressor gene among prostate cancer patients
Notice bibliographique
Résumé
Abstract MicroRNAs (miRNAs) are short, single-stranded RNA molecules with the ability of acting as post-transcriptional regulators of gene expression. MiRNAs regulate a number of important pathways, such as: cellular differentiation, proliferation, and apoptosis. MiRNA expression profiling patterns were demonstrated to have more potential than the common mRNA expression profiling for the characterization of poorly differentiated tumours. Studying miRNA expression profiles “signatures” will help in understanding the role of these miRNAs in the process of cancer development. The aim of this study was to profile the urinary miRNA expression signatures among prostate cancer patients in comparison to healthy male individuals. To identify the miRNA signatures specific for PCa, total RNA was isolated from 2.5mL of urine from all prostate cancer patients and from healthy males. RNA isolated from each group were pooled together and the differential expression analysis of miRNAs between the pooled samples was performed using MiRNA Microarray technology. Differential expression of two individual miRNAs, miR-1825 and miR-484, was observed between healthy males and prostate cancer patients. The relative expression of these two miRNAs were assessed among among the prostate cancer group and the healthy male group using RT-qPCR. MiR-1825 was up-regulated in seven out of eight PCa samples (88%), whereas miR-484 was down-regulated in six out of the eight PCa samples (75%). It has been found that the putative target for miR-1825 is member-1 of the Discoidin Domain family of Receptors (DDR1). The over-expression of DDR1 was reported to be a prognostic marker for the poor survival in several cancer types and therefore is proposed that miR-1825 might function as a tumor-suppressor by normally or in response to cancer, inhibiting DDR1's translation. As for miR-484, E3 ubiquitin-protein ligase (UBR5), which is thought to be involved with DNA repair, has been found to be the putative target for miR-484. It has be reported that the over-expression of a mutated version of UBR5 was found in breast and ovarian cancers where its deregulation was cited as a possible cause of malignant progression. It is therefore being proposed that miR-484 might function as a tumor- suppressor whereby its expression controls progression via the regulation of genes, such as UBR5, that have already been potentially associated with cancer progression. Citation Format: Moemen Abdalla, Taha A. Haj-Ahmad, Yousef Haj-Ahmad. Urinary miR-1825 and miR-484: An oncogene and a tumor- suppressor gene among prostate cancer patients. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3110. doi:10.1158/1538-7445.AM2015-3110
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».