Salvaging Genetic Material from Endangered Species: Xenografting of Testis Tissue from Immature Bison and Deer Donors into Recipient Mice Results in Complete Spermatogenesis.
Notice bibliographique
Résumé
The purpose of this study was to evaluate the long-term outcome of testis tissue xenografting from bison and deer as models for closely-related rare or endangered ungulates. Testis tissue was collected post-mortem from 2 newborn bison calves (Bison bison bison) and a 2-mo-old white-tailed deer fawn (Odocoileus virginianus). Small fragments of donor testis tissue were grafted under the back skin of immunodeficient recipient mice (n = 15 mice for bison xenografts and n = 7 mice for deer xenografts; 8 fragments/mouse). Single xenograft samples were removed from representative recipient mice every 2 mo from grafting for up to 16 mo for bison xenografts, and 14 mo post-grafting for deer xenografts. The retrieved xenografts were evaluated for seminiferous tubular density (per mm2) and tubular diameter, as well as for seminiferous tubular morphology and identification of the most advanced germ cell type present in each tubule cross section. Overall, 69% of the grafted testis fragments from donor bison and 63% of those from donor deer were recovered as xenografts. The weight of bison testis tissue xenografts increased (P < 0.02) ~4-fold by 2 mo and ~10-fold by 16 mo post-grafting. In bison xenografts, gradual maturational changes were evident in the form of seminiferous tubule expansion starting at 2 mo, first appearance of spermatocytes at 6 mo, round spermatids at 12 mo, and elongated spermatids at 16 mo post-grafting. For bison grafts, a donor effect on efficiency of spermatogenesis was also observed. Testis tissue xenografts from donor white-tailed deer also showed a gradual testicular development starting with tubular expansion also by 2 mo and presence of spermatocytes by 6 mo post-grafting, but round and elongated spermatids by 8 mo, followed by fully-formed spermatozoa by 12 mo post-grafting. The timing of complete spermatogenesis roughly corresponds to the reported timing of sexual maturation in these species. This study demonstrated, for the first time, that testis tissue xenografting from neonatal/immature bison or deer donors into recipient mice can successfully result in testicular maturation and development of spermatogenesis in the grafts up to the stage of spermatozoa production. These results may therefore provide a model for salvaging genetic material from immature males of rare or endangered ungulates that die prematurely. (platform)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».