Seminal Plasma Modulates Sperm Quality in the North American Bison (Bison bison) Across Breeding and Non-Breeding Seasons.
Notice bibliographique
Résumé
The overall goal of our research program is to develop methods to salvage the genetics of wood bison (Bison bison athabascae), a threathened species in Canada, using plains bison (Bison bison bison) as a model. The objective of the current study was to investigate the effects of reproductive seasonality on semen quality in bison bulls. In previous studies in our laboratory, sperm motility was significantly higher in the breeding versus non-breeding season for both wood and plains bison; however, there was no significant effect of season on motility of epididymal sperm from plains bison. Therefore, we hypothesized that composition of seminal plasma varied between breeding and non-breeding seasons, modulating sperm function. To test this hypothesis, protein profiles of seminal plasma were characterized with two-dimensional gel electrophoresis. There was differential expression of protein spots for both wood and plains bison across seasons. Using antibodies against proteins identified as bovine fertility markers, higher expression of BSP5 and TIMP-2 was confirmed in seminal plasma collected during the breeding season in wood and plains bison. To investigate the mechanism by which seminal plasma regulates sperm function, epididymal sperm from plains bison were mixed with seminal plasma collected from plains bison during either breeding or non-breeding seasons. Sperm were cryopreserved and subsequently evaluated. There was no significant difference between groups in sperm motility parameters (as determined by computer assisted sperm analysis). However, in sperm exposed to seminal plasma collected during the non-breeding season, tyrosine phosphorylation of sperm proteins (detected with immunoblotting) was increased; therefore, we inferred these sperm were more likely to undergo capacitation. In summary, there was differential expression of seminal plasma proteins across breeding and non-breeding seasons in North American bison, with higher expression of at least two bovine fertility-associated proteins during the breeding season. We concluded that seminal plasma-mediated modulation of sperm quality may contribute to reproductive seasonality in bison bulls. This research was supported by the Advancing Canadian Agriculture and Agri-Food Program, through the provincial councils of Alberta, Northwest Territories, Saskatchewan, Manitoba, Yukon and British Columbia (Project #AB0122CO), the Government of Northwest Territories, and the Calgary Zoo. (platform)
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».