Effect of the Luteinizing Hormone Surge on the RNA Expression of Granulosa Cells During Final Maturation of the Bovine Follicle.
Notice bibliographique
Résumé
During the mono-ovulatory estrus cycle, FSH and LH play an important role for growth, selection and dominance of the preovulatory follicle. The dominance of a follicle is characterized by the acquisition of luteinizing hormone (LH) receptors on granulosa cells (GC) and the transfer of its dependency for follicle-stimulating hormone (FSH) to LH. The LH surge induces profound changes in GC functions, which must be modified in a precise chronological order. These changes ultimately lead to the release of the matured oocyte during the follicular rupture at ovulation. On a transcriptional level, the gene expression cascades induced by this hormone, which lead to ovulation, are complex and still poorly understood. The objective of this study is to determine how the gene expression profile of GCs is modulated by the LH surge. The estrus of thirty animals were synchronized, they were then stimulated with FSH and finally the LH surge was induced by gonadotropin releasing hormone injection on day 14.5. Three different groups of GCs were collected by ovariectomy; (1) 2 h before the induction of the LH surge, (2) 6 h and (3) 22 h after the LH surge. The RNA of the different GC groups was extracted, amplified, labeled and hybridized on a microarray slide. A bovine oligonucleotide-spotted microarray (24K) was used to profile all known protein-encoding transcripts. The results obtained from the microarray data analysis showed that most transcripts were present in every type of GC studied. However, candidates were found to be preferentially expressed in only one of the GC states. Moreover, an unsupervised hierarchal clustering indicates that the overall GC expression of 2 h pre-LH and 6 h post LH correlate more closely than with the 22 h post LH, which is consistent with the endogenous ovarian physiological functions as GCs undergo extreme changes in order to achieve their luteal role. To understand the effect of LH on granulosa cell gene expression, transcripts fluctuating at the 6h post-LH stage were analyzed using pathway analysis software. Globally, the representation at 6 h post LH indicates that the follicles expresses genes involved in cell-to-cell interactions (like PTP4A3, THBS1, PPAP2B), in the inflammatory response (like B4GALT1, THBS1, TRAF3IP2) and in lipid metabolism modifications (like ACSL3, CYP51A1, FADS2). In conclusion, our goal is to put these follicular events in the context of oocyte developmental competence as it is gradually acquired during follicular growth and maximized during the last stages of follicular maturation. (poster)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,002 | 0,002 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».