Analysis of the Sperm Head Protein Profiles in Normozoospermic Men: Consistency Across Time in the Levels of Expression of Heat Shock Proteins and Peroxiredoxins.
Notice bibliographique
Résumé
The delivery of a genetically intact sperm nucleus during fertilization is required for normal embryo development. The condensed sperm DNA is closely associated with the proteinaceous sperm nuclear matrix. Although there is still little known about the functions of the nuclear matrix or its protein component, some nuclear proteins have been shown to have a role in normal sperm function. The objectives of this study were to identify the proteins in the head of human spermatozoa using a 1D Gel-MS label-free quantitative proteomics approach and to investigate quantitative and qualitative variations in the proteins extracted from human sperm heads. Sperm samples were obtained from each of three men (n=3) having high sperm counts at three different time points. This design allowed us to analyse intra-individual variations across time and inter-individual variations in the human sperm head proteome. To determine the background of the method used we analysed technical triplicates for each of the nine samples. The distribution of the coefficient of variation associated with the corresponding average of the triplicates was analysed. Intra-individual analysis from spectral counting methods reviewed that nearly 85% of the identified proteins had a low variation of expression level (< 2-fold) at various time points in each man. Across individuals, more than 30% of proteins were found to have a variation more than 2-fold with 10% considered highly variable (>3 fold). Interestingly, we found that 65 proteins did not vary across time or among men. This finding was confirmed both by ion profiling and spectral counting methods. Among these uniformly expressed proteins were chaperones, peroxiredoxins, isomerases, and cytoskeletal proteins. Our results suggest that there is a significant level of inter-individual variation in the protein profiles of human sperm heads. This highlights the importance of the effects of sample size and clinical characteristics of subjects when interpreting proteomic results with human sperm. The consistency in the levels of expression the various proteins suggests that they are essential and tightly regulated during spermatogenesis. Further investigations are required to elucidate their potential biological roles in sperm physiology. (Supported by the Canadian Institutes of Health Research - CIHR MOP86636).
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».