Caulobacter in cancer immunotherapy
Notice bibliographique
Résumé
A60 Purpose: To develop new cancer vaccine formulations using both the wild type (WT) and genetically modified Caulobacter crescentus (CC).Methods: Murine mammary carcinoma (EMT-6) cancer cell line was employed to investigate the anti-tumour effects of CC in two groups. In-vivo comparison of the effect of routes of administration on the antitumor effect of WT CC against EMT-6 was investigated. In-vivo comparison of the antitumor effects of the single and double mutant bacterial strains against EMT-6 was also performed. These include the CB15A KASC which is an S-layer minus strain, but still has the Smooth Lipopolysaccharides (LPS) and CB15A Ca5 KSAC which is a strain that has no S-layer and has a point mutation in the Smooth LPS. This latter strain can be referred to as the “naked” Caulobacter control. Natural killer (NK) cells from mice were isolated using magnetic activated cell separation from splenocytes using anti-NK cellanti-CD49b (DX5)microbead separator. Purified NK cells were co-incubated with WT CC, purified LPS, isolated S-layer protein ormouse interleukin-2 (IL-2).The cell-free supernatants from pooled cultures were collected for Enzyme Linked ImmunoSorbent Assay (ELISA) for interferon-gamma (IFN-γ) secretion. We developed a new active specific immunotherapy approach with high density display of MUC-1 breast cancer tandem peptide repeats on the S-Layer of CC.Results: WT treated mice showed a lower tumour volume with longer survival rate, when compared to the control group. Antitumor efficacy was higher in mice treated with intraperitoneal route than in intratumoral route of treatment. Normal saline treated and the double mutant control treated groups, exhibited high relative tumour volume and short survival rates compared to the WT bacteria and the single mutant strain treated groups. The latter showed a prolonged survival rates as well as reduced tumour volume. Thus, the results suggest that CC smooth LPS might be responsible for the bacteria anti-tumour activity. CC LPS directly stimulates pro-inflammatory cytokine IFN-γ production by NK cells. The in-vitro immunochemical characterization of the new designer high-density expression of multimeric-MUC-1 on CC display of cancer peptides using B27.29 monoclonal antibody against MUC-1 antigen using standard whole cell ELISA and SDS-PAGE analysis were established. Conclusion:CC may be a safe, bacterial immunomodulator for the treatment of tumors. The non-toxic and highly immunogenic CC-LPS play a key role in the inherent antitumor effect of the bacteria. Finally, CC is also valuable as a “platform” for particulate whole cell expression and presentation of MUC-1 peptide. Acknowledgement: The project is supported by the Natural Sciences and Engineering Research Council of Canada Collaborative Health Research Project (NSERC-CHRP).
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,003 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».